Prevalence of Listeria spp. in Traditional Indian Dairy Products from Chennai Metropolis, Tamil Nadu

Procedia food science Pub Date : 2016-01-01 DOI:10.1016/j.profoo.2016.02.025

S. Suriyapriya , P. Selvan , K. Porteen , S. Suresh Kannan

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引用次数: 6

Abstract

The present study was conducted between September, 2014 and June, 2015 to assess the prevalence of Listeria monocytogenes in Traditional Indian Dairy Products from Chennai, Tamil Nadu. Samples of Ghee, Rasagolla, Gulabjamun, Curd and Payasam were screened for the presence of L. monocytogenes using conventional culture method and validated by PCR. Among the 150 samples screened, 50 isolates from different dairy products (22 gulabjamun, 22 rasagolla and 06 curd samples) were presumptively identified as Listeria spp. Further confirmation by biochemical characterization and hemolysis on blood agar revealed that 34 isolates were Listeria welshimeri, 10 were L. murrayi and 06 were identified as L. seeligiri. The 34 isolates of L. welshimeri were present in 14 gulabjamun, 14 rasagolla and 06 curd samples. Similarly, 10 isolates of L. murrayi have been found in 04 gulabjamun and 06 rasagolla samples. L. seeligiri isolates were identified from 04 gulabjamun and 02 rasagolla samples. None of the Listeria species were found in ghee and payasam samples and interestingly, the results of both methods revealed that none of the traditional products screened were positive for L. monocytogenes

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泰米尔纳德邦金奈市传统印度乳制品中李斯特菌的流行情况

本研究于2014年9月至2015年6月期间进行，旨在评估来自泰米尔纳德邦金奈的传统印度乳制品中单核细胞增生李斯特菌的流行情况。采用常规培养法对Ghee、Rasagolla、Gulabjamun、Curd和Payasam样品进行单细胞增生乳杆菌的筛选和PCR验证。从150份不同乳制品样品中分离出50株李斯特菌(gulabjamun 22株、rasagolla 22株、凝乳06株)，经生化鉴定和血琼脂溶血实验证实，34株为威氏李斯特菌，10株为murrayi李斯特菌，06株为seeligiri李斯特菌。在14份古腊肠、14份拉萨瓜和06份凝乳中检出34株威氏乳杆菌。同样，在04份古拉巴jamun和06份rasagolla样品中发现了10株L. murrayi。从04份古拉贾蒙和02份沙芥样品中分离到seeligiri菌株。在酥油和payasam样品中均未发现李斯特菌，有趣的是，两种方法的结果均显示，筛选的传统产品均未检测到单核增生李斯特菌

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Procedia food science

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