Evaluation of indirect fluorescent antibody (IFA) test for kala-azar for diagnostic potential in endemic areas

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-02-01 DOI:10.1016/S0888-0786(96)80015-1

K. Mukerji , A. Puri , R. Sahai , R.P. Saxena , J.K. Srivastava , J.C. Katiyar , K.C. Saxena , B.N. Dhawan , B.B. Thakur

{"title":"Evaluation of indirect fluorescent antibody (IFA) test for kala-azar for diagnostic potential in endemic areas","authors":"K. Mukerji , A. Puri , R. Sahai , R.P. Saxena , J.K. Srivastava , J.C. Katiyar , K.C. Saxena , B.N. Dhawan , B.B. Thakur","doi":"10.1016/S0888-0786(96)80015-1","DOIUrl":null,"url":null,"abstract":"<div>An indirect fluorescent antibody (IFA) test using Leishmania donovani promastigote and amastigote antigens for diagnosis of kala-azar was evaluated in a kala-azar endemic area of Bihar, India. The test was found to be highly sensitive and specific using the promastigote antigen. It was positive in about 99% of parasitologically proven cases (n = 105), 89% of clinically suspected cases (n = 70) and all the sodium antimony gluconate (SAG) resistant cases (n = 80). None of the non-endemic control subjects (n = 40) or subjects infected with tuberculosis (n = 20), leprosy (n = 28), malaria (n = 20) and amoebiasis (n = 20) showed positive response. Only about 3% of the control subjects (n = 70) of the endemic area showed false positive response. Similar results were obtained with the amastigote antigen.</div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 1","pages":"Pages 9-12"},"PeriodicalIF":0.0000,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)80015-1","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Serodiagnosis and Immunotherapy in Infectious Disease","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0888078696800151","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 1

Abstract

An indirect fluorescent antibody (IFA) test using Leishmania donovani promastigote and amastigote antigens for diagnosis of kala-azar was evaluated in a kala-azar endemic area of Bihar, India. The test was found to be highly sensitive and specific using the promastigote antigen. It was positive in about 99% of parasitologically proven cases (n = 105), 89% of clinically suspected cases (n = 70) and all the sodium antimony gluconate (SAG) resistant cases (n = 80). None of the non-endemic control subjects (n = 40) or subjects infected with tuberculosis (n = 20), leprosy (n = 28), malaria (n = 20) and amoebiasis (n = 20) showed positive response. Only about 3% of the control subjects (n = 70) of the endemic area showed false positive response. Similar results were obtained with the amastigote antigen.

查看原文本刊更多论文

黑热病流行地区间接荧光抗体(IFA)检测诊断潜力的评价

在印度比哈尔邦黑热病流行区，对利用多诺瓦利什曼原虫原鞭毛菌和无鞭毛菌抗原诊断黑热病的间接荧光抗体(IFA)试验进行了评价。发现该试验使用promastigote抗原具有高度敏感性和特异性。约99%的寄生虫学证实病例(105例)、89%的临床疑似病例(70例)和所有葡萄糖酸锑钠(SAG)耐药病例(80例)呈阳性。非地方性对照(n = 40)和结核(n = 20)、麻风病(n = 28)、疟疾(n = 20)、阿米巴病(n = 20)感染者均无阳性反应。疫区仅有3%的对照(70例)出现假阳性反应。无尾线虫抗原也得到了类似的结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Serodiagnosis and Immunotherapy in Infectious Disease

自引率

0.00%

发文量