Two-Phase Medium – A New Approach to Microbiological Culturing

IF 1.4 4区生物学 Q3 MARINE & FRESHWATER BIOLOGY

Cryptogamie Algologie Pub Date : 2022-05-19 DOI:10.5252/cryptogamie-algologie2022v43a5

K. Wołowski, W. Spisak, A. Chlebicki, Mateusz Szar, J. Kozak, L. Śliwa

引用次数: 0

Abstract

ABSTRACT We tested a new two-phase culture medium for culturing microorganisms, in particular algae and fungi. In media composed of mixed gel and solid phases of granite, basalt, sandstone, limestone and sodium glass, we observed the growth of selected species inhabiting the boundaries of the gel medium with the solid phase. The species used were Klebsormidium dissectum (F.Gay) H.Ettl & Gärtner, Vischeria polyphem (Pitschmann) Kryvenda, Rybalka, Wolf & Friedl and Muriella decolor Vischer (algae); and Aspergillus tubingensis R.Mosseray, Aureobasidium pullulans (de Bary) G.Arnaud, Chaetomium globosum Kunze and Penicillium sp. Link (fungi). In the six-weeks period of parallel cultures, the growth rate of the algae inoculum in two-phase media was twice that observed in the control culture (gel medium). The algae also maintained high viability in the new medium.

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两相培养基——微生物培养的新途径

摘要:我们测试了一种新的两相培养基，用于培养微生物，特别是藻类和真菌。在由花岗岩、玄武岩、砂岩、石灰石和玻璃钠组成的凝胶固相混合介质中，我们观察到了居住在凝胶介质与固相边界上的精选物种的生长。所用种为解剖克雷伯sormidium dissectum (F.Gay) H.Ettl & Gärtner, polyphym Vischeria (Pitschmann) Kryvenda, Rybalka, Wolf & Friedl和Muriella decolor Vischer(藻类);tubingaspergillus R.Mosseray, auobasdium pululans (de Bary) g.a naud, Chaetomium globbosum Kunze和Penicillium sp. Link(真菌)。在6周的平行培养期间，两相培养基中接种的藻类生长速度是对照培养基(凝胶培养基)的两倍。藻类在新培养基中也保持了较高的活力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cryptogamie Algologie 生物-海洋与淡水生物学

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： Cryptogamie is a fast-track and peer-reviewed journal of international scope publishing in English only. It accepts original papers and review articles on the taxonomy, biology and ecology of all cryptogams. An issue of Cryptogamie may be devoted to a single topic, under the responsibility of guest editor(s). All articles published in Cryptogamie are compliant with the different nomenclatural codes. A copyright assignment will be signed by the authors before publication. Cryptogamie, Algologie accepts articles on systematics as well as ecology and evolution of any kind of algae (including Cyanobacteria).