Two-Phase Medium – A New Approach to Microbiological Culturing

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-05-19 DOI:10.5252/cryptogamie-algologie2022v43a5

K. Wołowski, W. Spisak, A. Chlebicki, Mateusz Szar, J. Kozak, L. Śliwa

引用次数: 0

Abstract

ABSTRACT We tested a new two-phase culture medium for culturing microorganisms, in particular algae and fungi. In media composed of mixed gel and solid phases of granite, basalt, sandstone, limestone and sodium glass, we observed the growth of selected species inhabiting the boundaries of the gel medium with the solid phase. The species used were Klebsormidium dissectum (F.Gay) H.Ettl & Gärtner, Vischeria polyphem (Pitschmann) Kryvenda, Rybalka, Wolf & Friedl and Muriella decolor Vischer (algae); and Aspergillus tubingensis R.Mosseray, Aureobasidium pullulans (de Bary) G.Arnaud, Chaetomium globosum Kunze and Penicillium sp. Link (fungi). In the six-weeks period of parallel cultures, the growth rate of the algae inoculum in two-phase media was twice that observed in the control culture (gel medium). The algae also maintained high viability in the new medium.

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两相培养基——微生物培养的新途径

摘要:我们测试了一种新的两相培养基，用于培养微生物，特别是藻类和真菌。在由花岗岩、玄武岩、砂岩、石灰石和玻璃钠组成的凝胶固相混合介质中，我们观察到了居住在凝胶介质与固相边界上的精选物种的生长。所用种为解剖克雷伯sormidium dissectum (F.Gay) H.Ettl & Gärtner, polyphym Vischeria (Pitschmann) Kryvenda, Rybalka, Wolf & Friedl和Muriella decolor Vischer(藻类);tubingaspergillus R.Mosseray, auobasdium pululans (de Bary) g.a naud, Chaetomium globbosum Kunze和Penicillium sp. Link(真菌)。在6周的平行培养期间，两相培养基中接种的藻类生长速度是对照培养基(凝胶培养基)的两倍。藻类在新培养基中也保持了较高的活力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.