Prevalence of Ureaplasma urealyticum in Endocervical Specimens of Female Patients in Qazvin, Iran

Biotechnology and Health Sciences Pub Date : 2016-11-15 DOI:10.17795/BHS-39599

H. Bahrami, T. Farivar, M. Aslanimehr, A. Peymani, Talat Dabaghi Ghaleh, H. Hashemi, Saman Saadat

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引用次数: 2

Abstract

Background: Ureaplasma urealyticum that is the smallest free-living bacterium does not have bacterial cell wall. These organisms cause different infections in respiratory and urinary tract system in close contact with epithelial cells. The current study aimed to determine the prevalence of U. urealyticum in endocervical samples of female patients referred to Kowsar hospital in Qazvin, Iran. Methods: The study was conducted on 232 married females aged 20 - 50 years. According to a gynecologist’s request, genital tract biopsies were taken from each individual using Dacron swabs. Each swab was placed into 4 mL of the pleural pneumonia-like organism (PPLO) broth media. Then 25 �L of the suspension was inoculated on the surface of PPLO agar. The remaining broth media and agar plates were incubated at 35°C in 5% CO2 atmosphere. Results: Out of 232 tested samples, 87 cases (37.5%) were positive for U. urealyticum and 145 (62.5%) were negative. Conclusions: Rapid laboratory detection of genital Ureaplasma in pregnant females is very important, mainly because of the ability of the bacteria to colonize the endocervical lining and cause injury to the fetus

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伊朗加兹温地区女性宫颈内膜标本解脲支原体的患病率

背景:解脲原体是最小的自由生活的细菌，没有细菌细胞壁。这些微生物与上皮细胞密切接触，可引起呼吸道和泌尿系统的不同感染。目前的研究旨在确定解脲杆菌在伊朗加兹温Kowsar医院转诊的女性患者宫颈内样本中的患病率。方法:对232名20 ~ 50岁的已婚女性进行调查。根据妇科医生的要求，用涤纶棉签对每个人进行了生殖道活检。将每个拭子放入4ml胸膜肺炎样菌(PPLO)肉汤培养基中。然后将25 μ L的悬浮液接种在PPLO琼脂表面。剩余的肉汤培养基和琼脂板在5% CO2气氛中35°C孵育。结果:232份检测样本中，解脲菌阳性87例(37.5%)，阴性145例(62.5%)。结论:妊娠女性生殖道脲原体的实验室快速检测具有重要意义，主要是由于该细菌能够在宫颈内膜上定植并对胎儿造成损伤

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Biotechnology and Health Sciences

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