Determination of the Dose of Erythrosine B as a Preparation for Inducing the Experimental Rats to Be Spastic

Modern clinical medicine research Pub Date : 2019-07-20 DOI:10.22606/MCMR.2019.32001

Marina Indriasari, Hardinsyah, L. Kustiyah, B. Priosoeryanto, F. H. Idris

引用次数: 1

Abstract

This study aimed to make the experimental rats spastic using Erythrosine B (ErB) in the correct dose. Five male Sprague-Dawley rats (T) aged 10-12 weeks with a weight of 200-250 mg were injected with ErB and doses as follows: 20 mg/kg bw for T1, 80 mg/kg bw for T2, 160 mg/kg bw for T3, 320 mg/kg bw for T4, and 400 mg/kg bw for T5. The results of 4-wk observation indicated that T1 had a rapid decrease in spasticity. T2 still experienced spasticity on day 3 with 100% muscle resistance. Its spasticity reduced by 25% within 14 days, and it still experienced spasticity with 50% muscle resistance within 28 days. T3 experienced spasticity with 100% muscle resistance until day 7. T4 and T5 died after induction. It could be concluded that the ErB dose of 80 mg/kg bw was used as a preparation for the induction of experimental rats.

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红素B作为实验性大鼠痉挛制剂的剂量测定

本研究旨在用正确剂量的红素B (ErB)使实验大鼠痉挛。5只10-12周龄雄性Sprague-Dawley大鼠(T)，体重200-250 mg，注射ErB，剂量分别为:T1 20 mg/kg bw, T2 80 mg/kg bw, T3 160 mg/kg bw, T4 320 mg/kg bw, T5 400 mg/kg bw。4周观察结果显示，T1组痉挛程度迅速下降。T2在第3天仍然出现痉挛，肌肉阻力100%。其痉挛性在14天内降低了25%，在28天内仍然出现痉挛，肌肉阻力为50%。T3经历痉挛，肌肉阻力100%，直到第7天。T4和T5诱导后死亡。由此可见，80 mg/kg bw的ErB剂量可作为诱导实验大鼠的制剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Modern clinical medicine research

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