Evaluating the long-term storage of Cryphonectria parasitica

Fungal Genetics Reports Pub Date : 2013-12-01 DOI:10.4148/1941-4765.1007

Joshua C. Springer, A. Baines, Matthew T. Chansler, A. Jarosz

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引用次数: 3

Abstract

Isolates of the Chestnut blight pathogen, Cryphonectria parasitica, from six populations in Michigan, were stored in the late 1990s as agar plugs of mycelium in vials of sterile water held at room temperature. Approximately 29% of the fungal isolates were infected with mycoviruses at the time of storage. Each isolate was tested for revivification effectiveness by taking aliquots from vials filled with agar plugs of C. parasitica and sterile water and plating onto potato dextrose agar. Average revivification success was 70.5% across populations with a range of 33—84% within populations. In situations where vials had dried out during storage, success was low (4%), while success for vials that retained sterile water averaged 90%. Most importantly however, is the loss of mycoviruses from stored isolates; only 2 of 119 stored mycovirus infected isolates still contained mycoviruses after storage, suggesting that the double-stranded RNA mycoviruses are degraded during storage. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol60/iss1/2

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褐飞虱的长期贮藏评价

板栗疫病病原菌Cryphonectria parasitica的分离株来自密歇根州的6个种群，在20世纪90年代末作为菌丝体的琼脂塞保存在室温无菌水的小瓶中。大约29%的真菌分离株在储存时感染了分枝病毒。每个分离株通过从装有寄生弧菌琼脂塞和无菌水的小瓶中取等份，并将其涂在马铃薯葡萄糖琼脂上，测试其复活效果。种群内平均恢复成功率为33-84%，种群间平均恢复成功率为70.5%。在储存期间小瓶干燥的情况下，成功率很低(4%)，而保留无菌水的小瓶成功率平均为90%。然而，最重要的是，从储存的分离株中丢失了分枝病毒;保存的119株分枝病毒感染株中只有2株在保存后仍含有分枝病毒，表明双链RNA分枝病毒在保存过程中被降解。本作品采用知识共享署名-相同方式共享4.0许可协议。这篇常规论文可在真菌遗传学报告:http://newprairiepress.org/fgr/vol60/iss1/2

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Fungal Genetics Reports

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