Extraction of Stevioside and Rebaudioside A from Stevia rebaudiana by percolation with ethanol-water solvents

Abstract

The aim of this study was to analyze the optimum temperature and percentage of ethanol to extract steviol glycosides (Stevioside and Rebaudioside A) from stevia leaves in a percolation extractor. The experiments were performed in broad ranges of binary solvent (0-100 % ethanol) and temperature (30–70 °C) in order to investigate their effects on steviol glycosides recovery and purity. The optimal extraction conditions, which gave a best steviol glycosides extraction kinetic were 70 °C and 35% of ethanol. In addition, the optimal extraction condition giving maximum purity was 70% of ethanol between 30 and 70°C.