Anticancer Activity of Biosynthesized Silver Nanoparticles using Murraya koenigii Leaf Extract against HT-29 Colon Cancer Cell Line

Abstract

Plants extract from Murraya koenigii was used for the synthesis of silver nanoparticles (Ag NPs) using silver nitrate solution. Ag NPs were characterized by UV–vis spectrophotometer, scanning electron microscope (SEM), Energy Dispersive Spectroscopy (EDX) and Fourier Transform Infra-Red Spectroscopy (FT-IR). The formation of stable silver nanoparticles reduced to the colloidal solution are observed by UV–vis spectrophotometer analysis. SEM determination of the brown coloured samples with well dispersed nanoparticles seen after treatment with silver nitrate showed the presence of silver nanoparticle whereas the EDX analysis performed is to confirm the presence of silver molecules in the sample and FTIR measurement carried out identifies the biomolecules present in M. koenigii leaf responsible for capping leading to efficient stabilization of the silver nanoparticles. The anticancer potential of the nanoparticles was evaluated using MTT assay on HT-29 colon cancer cell line. Ag NPs showed potent cytotoxic activity against the human colorectal adenocarcinoma (HT-29) cell line at higher concentrations. This study insights the M. koenigii synthesized silver NP’s could be an effective applicability drug candidate for colon cancer.