Changes in the indicators of immunobiochemical blood status of cows during correction of the functional activity of the ovaries with a drug based on GM-CSF

Abstract

The state of the immunobiochemical status of the blood of cows when using a drug containing gm-csf was studied on 15 animals with a hypofunctional state of the ovaries. The animals were divided according to the principle of analogues into three groups. The animals were included in the experiment 45- 60 days after calving without signs of sexual cyclicity. The cows of the first group were injected with a drug based on gm-csf (granulocyte-macrophage colony-stimulating factor), the cows of the second one - follimag, the third group served as a negative control without the introduction of drugs. Evaluation of the efficacy of the use of gonadotropic and recombinant drugs showed that the use of the agent based on gm-csf turned out to be the most effective in comparison with follimag and the negative control. After the use of the drug of recombinant interferons, the content of circulating immune complexes turned out to be lower by 30.9%, malon dialdehyde - by 17.1%, medium molecular peptides - by 34.9% TNFα - by 21.0%, IL-1β - by 41.1%, IL-2 - by 35.9%, with an increase in serum bactericidal and lysozyme activity by 13.8% and 21.7%, respectively, phagocytic index - by 12.9%, phagocytic number - by 18.4%, progesterone - by 5.09 times, estradiol - by 29.3%, dehydroepiandrosterone sulfate - by 78.9%, IL-4 - by 44.6%, IL-10 - by 20.3%, which indicates activation the humoral link of general nonspecific resistance, an increase in the content of anti-inflammatory cytokines, with a decrease in the intensity of lipid peroxidation processes, the level of proinflammatory cytokines against the background of the restoration of the hormoneproducing function of the sex gonads. Changes in the indicators of the immunobiochemical blood status of cows are reflected in the activation of the functional activity of the ovaries, the manifestation of full-fledged sexual cycles by animals and successful insemination.