Dental applications of induced pluripotent stem cells and their derivatives

IF 5.7 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Japanese Dental Science Review Pub Date : 2022-11-01 DOI:10.1016/j.jdsr.2022.03.002

Pan Gao , Shan Liu , Xiaoyi Wang , Makoto Ikeya

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引用次数: 1

Abstract

Periodontal tissue regeneration is the ideal tactic for treating periodontitis. Tooth regeneration is the potential strategy to restore the lost teeth. With infinite self-renewal, broad differentiation potential, and less ethical issues than embryonic stem cells, induced pluripotent stem cells (iPSCs) are promising cell resource for periodontal and tooth regeneration. This review summarized the optimized technologies of generating iPSC lines and application of iPSC derivatives, which reduce the risk of tumorigenicity. Given that iPSCs may have epigenetic memory from the donor tissue and tend to differentiate into lineages along with the donor cells, iPSCs derived from dental tissues may benefit for personalized dental application. Neural crest cells (NCCs) and mesenchymal stem or stomal cells (MSCs) are lineage-specific progenitor cells derived from iPSCs and can differentiate into multilineage cell types. This review introduced the updated technologies of inducing iPSC-derived NCCs and iPSC-derived MSCs and their application in periodontal and tooth regeneration. Given the complexity of periodontal tissues and teeth, it is crucial to elucidate the integrated mechanisms of all constitutive cells and the spatio-temporal interactions among them to generate structural periodontal tissues and functional teeth. Thus, more sophisticated studies in vitro and in vivo and even preclinical investigations need to be conducted.

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诱导多能干细胞及其衍生物在牙科的应用

牙周组织再生是治疗牙周炎的理想策略。牙齿再生是修复牙齿缺失的潜在策略。与胚胎干细胞相比，诱导多能干细胞具有无限的自我更新能力、广泛的分化潜力和较少的伦理问题，是牙周和牙齿再生的理想细胞资源。本文综述了降低致瘤性风险的诱导多能干细胞系的优化制备技术及诱导多能干细胞衍生物的应用。鉴于iPSCs可能具有来自供体组织的表观遗传记忆，并倾向于与供体细胞一起分化成谱系，来自牙科组织的iPSCs可能有利于个性化牙科应用。神经嵴细胞(NCCs)和间充质干细胞或stomal细胞(MSCs)是来源于ipsc的谱系特异性祖细胞，可以分化为多谱系细胞类型。本文综述了诱导ipsc来源的ncc和MSCs的最新技术及其在牙周和牙齿再生中的应用。考虑到牙周组织和牙齿的复杂性，阐明所有组成细胞的综合机制以及它们之间的时空相互作用对于形成结构性牙周组织和功能牙齿至关重要。因此，需要进行更复杂的体外和体内研究，甚至临床前研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Japanese Dental Science Review DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

9.90

自引率

1.50%

发文量

审稿时长

32 days

期刊介绍： The Japanese Dental Science Review is published by the Japanese Association for Dental Science aiming to introduce the modern aspects of the dental basic and clinical sciences in Japan, and to share and discuss the update information with foreign researchers and dentists for further development of dentistry. In principle, papers are written and submitted on the invitation of one of the Editors, although the Editors would be glad to receive suggestions. Proposals for review articles should be sent by the authors to one of the Editors by e-mail. All submitted papers are subject to the peer- refereeing process.