Preparation of liposomal nanocarrier by extruder to enhance tumor accumulation of paclitaxel

IF 2.1 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Bioactive and Compatible Polymers Pub Date : 2021-11-05 DOI:10.1177/08839115211053926

Ngoc Thuy Trang Le, N. H. Nguyen, Minh Chau Hoang, Cuu Khoa Nguyen, Dai Hai Nguyen, Dieu Linh Tran

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引用次数: 4

Abstract

Despite the wide-spectrum and effective anti-cancer activity of paclitaxel (PTX), their low solubility and side effects are the main challenges in their clinical application. In this study, a model paclitaxel-encapsulated nanoliposome (NLips-PTX) carrier was synthesized to enhance PTX solubility and increase its passive accumulation at the tumor site. Soy lecithin and cholesterol at a 9:1 ratio were used to prepare the nano-sized liposomes through the thin-film hydration followed by extrusion technique. The prepared spherical NLips-PTX liposomes with an average size of about 150 nm and high uniformity were characterized by DLS and TEM. PTX load efficiency of NLips was determined at about 85% by HPLC. NLips-PTX also showed a therapeutic effect toward breast cancer cells (MCF-7) in a dose- and time-dependent manner via in vitro cellular uptake and a cytotoxicity study. This research indicates that extrusion is a simple and convenient method for nano-sizing and homogenising liposome suspension for potentially effective delivery of drug to target tumor sites.

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挤压法制备脂质体纳米载体促进紫杉醇肿瘤蓄积

紫杉醇(PTX)具有广谱、有效的抗癌活性，但其低溶解度和副作用是其临床应用的主要挑战。本研究合成了一种紫杉醇包封纳米脂质体(NLips-PTX)载体模型，以增强PTX的溶解度，增加其在肿瘤部位的被动蓄积。以大豆卵磷脂与胆固醇的比例为9:1，采用薄膜水化后挤压法制备纳米脂质体。制备的球形NLips-PTX脂质体平均粒径约为150 nm，均匀度高，通过DLS和TEM对其进行了表征。高效液相色谱法测定NLips的PTX负载效率约为85%。通过体外细胞摄取和细胞毒性研究，NLips-PTX对乳腺癌细胞(MCF-7)也显示出剂量和时间依赖性的治疗效果。该研究表明，挤压是一种简单方便的纳米化和均质脂质体悬浮液的方法，可以有效地将药物输送到目标肿瘤部位。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Bioactive and Compatible Polymers 工程技术-材料科学：生物材料

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： The use and importance of biomedical polymers, especially in pharmacology, is growing rapidly. The Journal of Bioactive and Compatible Polymers is a fully peer-reviewed scholarly journal that provides biomedical polymer scientists and researchers with new information on important advances in this field. Examples of specific areas of interest to the journal include: polymeric drugs and drug design; polymeric functionalization and structures related to biological activity or compatibility; natural polymer modification to achieve specific biological activity or compatibility; enzyme modelling by polymers; membranes for biological use; liposome stabilization and cell modeling. This journal is a member of the Committee on Publication Ethics (COPE).