Differentiation of mycelia and basidiomes of Antrodia cinnamomea using certain chemical components.

Abstract

Using 10 known components in Antrodia cinnamomea including 5 ergostanes (antcins C and K, and zhankuic acids A, B, and C), 4 lanostanes (sulphurenic acid, dehydrosulphurenic acid, eburicoic acid, and dehydroeburicoic acid), and 1 monophenyl (4,7-dimethoxy-5-methyl-1,3-benzodioxole) as standards, mycelia and basidiomes of A. cinnamomea were differentiated in this study. Natural basidiomes collected from wood of Cinnamomum kanehirai in natural forests and cultured basidiomes grown on potato dextrose agar medium contained all 10 test components. However, natural mycelia collected from the wood of C. kanehirai in a natural forest and liquid/solid cultured mycelia grown on potato dextrose broth/potato dextrose agar media contained the 4 lanostanes and 4,7-dimethoxy-5-methyl-1,3-benzodioxole but not the 5 ergostanes. These results indicate that the production of ergostanes is related to basidiomatal formation of A. cinnamomea, but is not related to the substrate on which the organism is grown.