Molecular characterization of Klebsiella pneumoniae associted with Thalassemia in Thi-Qar Governorate

R. Joudah, S. Hamim
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Abstract

Klebsiella pneumoniae is a gram-negative, aerobic, non-motile bacilli and is a common cause of a wide range of infections in humans, Klebsiella spp. as a major causative bacteria in Thalassemia patient. The present study aimed to shed light on the supposed  role of bacterial infection in Thalassemia patients during the period of from August 2022 to December 2023 in Genetic Blood Diseases/Thi-Qar Governorate. Forty blood sample were collected from all patients and subjected to Conventional Polymerase Chain Reaction assay by using the Universal gene. Nine PCR product were selected and subjected to partial DNA sequencing for the 16S rRNA gene to follow up their possible relationship between them and what recorded globally in Gene bank.                       The results revealed that 9/40(25%) isolates were                     K. pneumoniae. PCR product of 16SrRNA registered in Gene bank under the official accession numbers of OQ928953.1,OQ929666.1,OQ928950.1,OQ929250.1,OQ927231.1,OQ928942.1,OQ927249.1,OQ927229.1 and    OQ927253.1).                                                                     The phylogenetic tree that was constructed by MEGA-10 software showed that there were different molecular relationships among the local K. pneumonia  isolates with analogous ones around the world.
卡塔尔省与地中海贫血相关的肺炎克雷伯菌分子特征
肺炎克雷伯菌是一种革兰氏阴性、需氧、非运动杆菌,是人类广泛感染的常见原因,克雷伯菌属是地中海贫血患者的主要致病菌。本研究旨在阐明2022年8月至2023年12月遗传血友病/Thi-Qar省地中海贫血患者中细菌感染的假设作用。所有患者采集40份血样,采用通用基因进行常规聚合酶链反应测定。选取9个PCR产物,对其进行16S rRNA基因的部分DNA测序,以追踪它们与基因库中全球记录的基因之间可能存在的关系。结果显示,9/40(25%)的隔离                      肺炎。16 srrna注册基因的PCR产物加入银行根据官方数字OQ928953.1, OQ929666.1, OQ928950.1, OQ929250.1, OQ927231.1, OQ928942.1, OQ927249.1, OQ927229.1 OQ927253.1 ).                                                                     利用MEGA-10软件构建的系统发育树显示,本地分离的肺炎克雷伯菌与世界各地的类似分离的肺炎克雷伯菌存在不同的分子关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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