Antioxidant Activity of Constituents from the Methanolic Extract of Acacia confusa Leaves

Q4 Agricultural and Biological Sciences

Taiwan Journal of Forest Science Pub Date : 2009-03-01 DOI:10.7075/TJFS.200903.0061

Shaw-Shien Lin, I. Shiau, Shang-Tzen Chang

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引用次数: 3

Abstract

In this study, the antioxidant activities of the leaf extract of Acacia confusa and constituents isolated from its ethyl acetate (EtOAc) fraction were investigated for the first time using various in vitro assays. Ten antioxidant compounds, namely, 3,4,5-trihydroxybenzoic acid methyl ester (1), 5,7,3',4'-tetrahydroxyl-flavone (2), myricetin 3-O-(3”-O-galloyl)-α-rhamnopyranoside (3), myricetin 3-O-(3”-O-galloyl)-α-rhamnopyranoside 7-methyl ether (4), myricetin 3-O-(2”-O-galloyl)-α-rhamnopyranoside 7-methyl ether (5), myricetin-3-O-β-glucopyranoside (6), myricetin-3-O-α-rhamnopyranoside (7), myricetin 3-O-(2”-O-galloyl)-α-rhamnopyranoside (8), quercetin-3-O-α-rhamnopyranoside (9), and europetin-3-O-α-rhamnopyranoside (10) were isolated and identified from the leaf extract. In addition, their 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity, superoxide radical-scavenging activity, and trolox equivalent antioxidant capacity (TEAC) were compared with catechin (as the positive control). As to the DPPH-scavenging activity, all compounds except for 1 and 2 displayed greater antioxidant capacities than catechin (EC50=10.3 μM), and all of their TEAC values were higher than that of catechin (3.49 mM of trolox) except for 1, 6, 9, and 10. In addition, all compounds except for 1, 2, 5, and 6, also expressed as high a superoxide radical scavenging activity as catechin (EC50=7.4 μM).

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金合欢叶甲醇提取物成分的抗氧化活性研究

本研究首次采用各种体外实验方法研究了金合欢叶提取物及其乙酸乙酯(EtOAc)组分的抗氧化活性。十种抗氧化化合物，分别为3,4,5-三羟基苯甲酸甲酯(1)、5,7,3'，4'-四羟基黄酮(2)、杨梅素3- o -(3 '- o -没食子基)-α-鼠李核苷(3)、杨梅素3- o -(3 '- o -没食子基)-α-鼠李核苷7-甲基醚(4)、杨梅素3- o -(2 '- o -没食子基)-α-鼠李核苷7-甲基醚(5)、杨梅素3- o -β-葡萄糖苷(6)、杨梅素3- o -(2 '- o -没食子基)-α-鼠李核苷(7)、杨梅素3- o -(2 '- o -没食子基)-α-鼠李核苷(8)、槲皮素3- o -α-鼠李核苷(9)、从其叶提取物中分离鉴定出欧洲素-3- o -α-鼠李糖苷(10)。此外，以儿茶素为阳性对照，比较了其1,1-二苯基-2-苦基肼基(DPPH)自由基清除活性、超氧化物自由基清除活性和trolox等效抗氧化能力(TEAC)。在dpph清除活性方面，除1和2外，其余化合物的抗氧化能力均高于儿茶素(EC50=10.3 μM);除1、6、9和10外，其余化合物的TEAC值均高于儿茶素(3.49 mM的trolox)。此外，除1、2、5和6外，其余化合物的超氧自由基清除活性均与儿茶素相当(EC50=7.4 μM)。

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来源期刊

Taiwan Journal of Forest Science Agricultural and Biological Sciences-Forestry

CiteScore

0.20

自引率

0.00%

发文量

期刊介绍： The Taiwan Journal of Forest Science is an academic publication that welcomes contributions from around the world. The journal covers all aspects of forest research, both basic and applied, including Forest Biology and Ecology (tree breeding, silviculture, soils, etc.), Forest Management (watershed management, forest pests and diseases, forest fire, wildlife, recreation, etc.), Biotechnology, and Wood Science. Manuscripts acceptable to the journal include (1) research papers, (2) research notes, (3) review articles, and (4) monographs. A research note differs from a research paper in its scope which is less-comprehensive, yet it contains important information. In other words, a research note offers an innovative perspective or new discovery which is worthy of early disclosure.