Experimental evaluation of beneficial potential of Rhizophora mucronata Lam. leaves in Cisplatin-induced nephrotoxic animal model

Abstract

Objective: Chronic kidney disease typically evolves over many years, with a long latent period when the disease is clinically silent. Diagnosis, progression and treatment of renal impairment are based mainly on biomarkers that assess kidney function. There are a number of drugs used in the therapeutics which possesses nephrotoxic properties. But till date there is no nephroprotective drug available in therapeutics. In recent years the popularity of the herbal drugs is increased for their safety, efficacy and cost effectiveness. They have also gain importance in the drug development research. Present study has been focused to evaluate the preventive effect of the ethanolic extract of Sunderban mangrove Rhizophora mucronata Lam. leaves (RME) against cisplatin induced nephrotoxicity in experimental animal model. Methods: In-vivo nephrotoxic model was developed using a single dose of Cisplatin (5mg/kg i.p.) in rats. The extract (RME) was administered in different doses orally for consecutive 14 days and different biochemical parameters were evaluated at the end of the study. Results: The extract (RME) significantly reduced the urine output, kidney weight, urinary microprotein, microalbumin, elevated serum urea, creatinine levels than the nephrotoxic control rats. RME at the doses 200mg/kg and 400mg/kg body weight orally also had significant nephroprotective activity. Conclusion: Present study results indicated the protective effect of the ethanolic extract of the mangrove Rhizophora mucronata Lam. leaves in Cisplatin induced nephrotoxic model in rats. The amelioration of the nephrotoxicity might be mediated by the secondary metabolites of the plant extract, possessing significant antioxidant property.