Antibacterial activity of pigment produced from Micrococcus luteus KF532949

Abstract

Aim

The problems of synthetic pigment causing toxicity and carcinogenicity in the human body decrease its use. Therefore, interest in natural pigment production is increasing. Therefore, the marine environment has recently become an attractive research subject for many investigations, because of its rich biodiversity. Attempt has been initiated to isolate Micrococcus luteus from marina beach water.

Methods

The sea water sample isolated was allowed to grow in a Zobell marine agar medium and incubated at 37 °C for one week. The isolated strain from marine source was confirmed by 16S rRNA sequencing. The GC content was found to be 58.6%. Phylogenetic tree analysis was performed to know the sequences that are closely related. The strain was assessed for pigment production in Zobell marine broth and incubated at 37 °C for two days. The swab sticks collected from hospital were streaked directly on the labelled agar plates such as eosin methylene blue, MacConkey, nutrient, blood, mannitol salt agar and incubated at 37 °C for 24 h and characterized. The antibacterial activity of crude pigment was tested against wound isolates.

Results

The individual yellow colour colonies obtained was sequenced, submitted to gene bank under the accession number KF532949. Phylogenetic analysis revealed, that the identified strain M. luteus is closely related to various Micrococcus sp. The crude pigment showed promising results against Staphylococcus sp., Klebsiella sp., Pseudomonas sp. isolated from wound.

Conclusion

From this result, we can conclude that the isolated strain M. luteus is able to act against both gram positive and gram negative bacteria.