Assessing the Single and Combined Toxicity of Chlorantraniliprole with Bacillus thuringiensis against Maize Fall Armyworm Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) under Laboratory Conditions

V. Gowtham, M. Muthuswami, N. Sathiah, S. Geetha, S. Varanavasiappan, D. Uma
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引用次数: 2

Abstract

Use of synthetic insecticides for the management of fall armyworm (FAW) Spodoptera frugiperda (J. E. Smith) for a longer period will led to development of insecticide resistance. Identification of an eco-friendly synergistic agent to enhance the toxicity potential and reduced pesticide use as well become mandatory in due process. Hence the present study was formulated to find the single and combined toxicity of chlorantraniliprole and Bacillus thuringiensis (Bt) against the 2nd and 3rd larval instars of S. frugiperda. Single toxicity of chlorantraniliprole against 2nd and 3rd larval instars were 0.87 and 1.52 ppm (LC25); 4.08 and 6.50 ppm (LC50), respectively. With respect to Bt, single toxicity against 2nd and 3rd larval instars were 474.39 and 693.48 ppm (LC25); 1008.62 and 1228.62 ppm (LC50), respectively. Combination effect of chlorantraniliprole with Bt revealed that 2nd instar of FAW showed supplemental synergism at LC50 of chlorantraniliprole + LC25 of Bt. In the case of LC50 of chlorantraniliprole + LC50 of Bt, LC25 of chlorantraniliprole + LC50 of Bt and LC25 of chlorantraniliprole + LC50 of Bt combinations, they showed sub additive synergism. In 3rd instar larvae, the combined toxicity results were similar for all the combinations of chlorantraniliprole + Bt except LC25 of chlorantraniliprole + LC50 of Bt where it showed an antagonistic synergism. Activity of Carboxyl Esterase (CarE), Mixed Function Oxidase (MFO) and Glutathione-S-Transferase (GST) were found to be lesser in chlorantraniliprole LC50 + Bt LC25 combinations than single toxicity treatments. Therefore, combined use of chlorantraniliprole with Bt at LC50 of chlorantraniliprole + LC25 of Bt had supplemental synergism on fall armyworm under laboratory condition.
氯虫腈与苏云金芽孢杆菌单用及联用对玉米秋粘虫(鳞翅目:夜蛾科)的毒性研究
长时间使用合成杀虫剂管理秋粘虫(Spodoptera frugiperda, j.e. Smith)将导致抗药性的产生。确定一种生态友好的增效剂,以提高潜在毒性和减少农药的使用,并在适当的程序中成为强制性的。因此,本研究旨在研究氯虫腈和苏云金芽孢杆菌(Bacillus thuringiensis, Bt)对frugiperda 2龄和3龄幼虫的单独和联合毒性。氯虫腈对2、3龄幼虫的单次毒力分别为0.87、1.52 ppm (LC25);分别为4.08和6.50 ppm (LC50)。对2龄和3龄幼虫单毒分别为474.39和693.48 ppm (LC25);1008.62和1228.62 ppm (LC50)。氯虫腈与Bt的联合效应显示,2龄FAW在氯虫腈LC50 + Bt LC25处表现出互补增效作用,在氯虫腈LC50 + Bt LC50、氯虫腈LC25 + Bt LC50、氯虫腈LC25 + Bt LC50、氯虫腈LC25 + Bt LC50组合的情况下表现为亚加性增效作用。在3龄幼虫中,除氯虫腈+ Bt的LC25 + LC50表现出拮抗协同作用外,氯虫腈+ Bt的联合毒力结果基本一致。氯虫腈LC50 + Bt LC25联合处理的羧基酯酶(CarE)、混合功能氧化酶(MFO)和谷胱甘肽- s -转移酶(GST)活性低于单毒处理。因此,在实验室条件下,氯虫腈与Bt在氯虫腈LC50 + Bt LC25下联合施用对秋粘虫具有补充增效作用。
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