In vitro effects of Sunset Yellow on Chromosomal Damage and Sister Chromatid Exchanges in Human Peripheral Lymphocytes

A. Algarni
{"title":"In vitro effects of Sunset Yellow on Chromosomal Damage and Sister Chromatid Exchanges in Human Peripheral Lymphocytes","authors":"A. Algarni","doi":"10.9734/arrb/2021/v36i1230466","DOIUrl":null,"url":null,"abstract":"Sunset Yellow (SY) is an organic azo dye that is used extensively as a coloring agent in many industries, such as cosmetics, pharmaceuticals ,and foodstuffs. Many studies have conflicting results about the genotoxicity effect of SY. Thus, the purpose of this study was to provide additional data concerning SY genotoxicity in human lymphocytes by using chromosomal aberrations (CAs) and sister chromatid exchanges (SCEs) assay. Four concentrations of Sunset Yellow (1, 5, 20 ,and 50 mg/ml) were used on human lymphocyte cultures. Positive and negative controls were mitomycin C and distilled water, respectively. Compared to the control, SY caused a significant increase in CAs and SCEs frequencies at all concentrations. A total of five types of CAs were observed, such as gaps, fragments, RCF, stickiness,and polyploidy. According to the present results, high concentrations of SY are genotoxic in vitro to cultured human lymphocytes. To determine its full genotoxicity potential, SY should be tested in other test systems.","PeriodicalId":8230,"journal":{"name":"Annual research & review in biology","volume":"41 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annual research & review in biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9734/arrb/2021/v36i1230466","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2

Abstract

Sunset Yellow (SY) is an organic azo dye that is used extensively as a coloring agent in many industries, such as cosmetics, pharmaceuticals ,and foodstuffs. Many studies have conflicting results about the genotoxicity effect of SY. Thus, the purpose of this study was to provide additional data concerning SY genotoxicity in human lymphocytes by using chromosomal aberrations (CAs) and sister chromatid exchanges (SCEs) assay. Four concentrations of Sunset Yellow (1, 5, 20 ,and 50 mg/ml) were used on human lymphocyte cultures. Positive and negative controls were mitomycin C and distilled water, respectively. Compared to the control, SY caused a significant increase in CAs and SCEs frequencies at all concentrations. A total of five types of CAs were observed, such as gaps, fragments, RCF, stickiness,and polyploidy. According to the present results, high concentrations of SY are genotoxic in vitro to cultured human lymphocytes. To determine its full genotoxicity potential, SY should be tested in other test systems.
日落黄对人外周血淋巴细胞染色体损伤及姐妹染色单体交换的体外影响
日落黄(SY)是一种有机偶氮染料,广泛用作许多行业的着色剂,如化妆品,药品和食品。许多研究对SY的遗传毒性作用有相互矛盾的结果。因此,本研究的目的是通过染色体畸变(CAs)和姐妹染色单体交换(SCEs)试验,提供关于SY对人淋巴细胞遗传毒性的额外数据。四种浓度的日落黄(1、5、20和50 mg/ml)用于人淋巴细胞培养。阳性对照为丝裂霉素C,阴性对照为蒸馏水。与对照组相比,在所有浓度下,SY引起CAs和sce频率显著增加。共观察到间隙、片段、RCF、黏性、多倍体等5种类型的ca。根据目前的结果,高浓度的SY对体外培养的人淋巴细胞具有遗传毒性。为确定其全部遗传毒性潜力,应在其他测试系统中测试SY。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信