SEASONAL VARIATIONS IN ANTIOXIDANT CAPACITIES AND PHENOLIC CONTENTS OF TEA LEAF EXTRACTS

Abstract

Objectives: The objective of the study was to estimate the seasonal variations in the antioxidant capacities, total polyphenol content (TPC), total flavonoid content (TFC), and tannin content (TC) of tea leaf extracts from two different plantation sites. Methods: Samples were collected from two tea gardens in Tuli and Ungma situated at N 26°39’19.3 E 094°39’22.7 and N 26°17’30.6 E 094°28’29.2, respectively, under the Mokokchung district of Nagaland, India. TPC, TFC, and TC from sample extracts were determined using Folin–Ciocalteu reagent, aluminum chloride colorimetric, and Folin–Ciocalteu assay. Apart from these, antioxidant capacities were analyzed using ferric reducing ability of plasma (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Results: The concentrations of total polyphenol, flavonoid, and tannin varied from 552.029±8.079 to 305.647±1.744 mg gallic acid equivalent/g, 238.770±0.508–148.457±1.653 mg catechin equivalent/g, and 26.453±0.485–20.173±0.173 mg tannic acid equivalent/g, respectively. FRAP and DPPH assay displayed value ranging from 2.564±0.023 to 1.074±0.023 mmol Fe(II) equivalent/g and 3.612±0.053–2.076±0.028 mmol Trolox equivalent/g. Significant seasonal variations in concentrations of these compounds were observed and a positive correlation between antioxidant capacities and phenolics of tea leaf extracts was established. Conclusion: Tea (Camellia sinensis (L.) O. Kuntze) has been regarded as a plant of immense medicinal and therapeutic value since time immemorial. The tea leaf extracts analyzed in this study gave high TPC, TFC, and TC, as well as high antioxidant activity in terms of DPPH and FRAP value. Studying such properties in tea leaves contributes more to our understandings of health benefit potentials in tea leaves and the quality of tea leaves on the basis of seasons and sites where they are planted.