Efektivitas Daya Hambat Ekstrak Etanol 96% Bonggol Nanas (Ananas Comosus L) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus

Umar udin, Rinda Yunia Sari, S. Anto
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引用次数: 6

Abstract

ABSTRAKBonggol belum dimanfaatkan secara optimal, padahal bagian bonggol mengandung beberapa komponen aktif salah satunya adalah enzim bromelin. Ezim bromelin bersifat sebagai antibakteri. Tujuan penelitian ini  untuk mengetahui efektivitas daya hambat ekstrak etanol 96% bonggol nanas (Ananas comosus L) terhadap perumbuhan bakteri Staphylococcus aureus. Penelitian ini merupakan penelitian eksperimental menggunakan desain post-test only control group design. Ekstrak etanol 96% bonggol nanas (Ananas comosus L) dilaukan ekstra dengan metode maserasi Bakteri Staphycoccus aureus diambil dari media Manitol salt agar. Hasil penelitian ini menunjukkan ekstrak bonggol nanas pada konsentrasi 50%-100% dengan diulang sebanyak 3 replikasi memiliki daya hambat terhadap pertumbuhan bakteri Staphylococcus aureus. Berdasarkan uji One-Way ANOVA dengan taraf kepercayaan 95% menunjukkan terdapat perbedaan yang bermakna (p<0,05) antara berbagai konsentrasi, sehingga dilanjutkan dengan uji LSD untuk mengetahui adanya perbedaan pada masing-masing perlakuan. Hasil uji LSD menunjukkan bahwa kosentrasi 50% berbeda nyata dengan konsentrasi  70%, 80%, 90% dan 100%, sedangkan control positif tidak berbeda nyata dengan konsentrasi 50% dan 60%. Kesimpulan penelitian ini bahwa ekstrak etanol 96% bonggol nanas (Ananas comosus L) berpengaruh positif dalam menghambat dan membunuh bakteri Staphylococcus aureus dan konsentrasi paling optimal pada 70%.Kata kunci: Ekstrak etanol 96% bonggol nanas, bacitrasin, Staphylococcus aureus, zona hambat antibakteriABSTRACTPineaplle cobs have not been utilized optimally, whereas the cob section contains several active components one of which is the enzyme bromelin.  Bromelin is an antibacterial agent.  The purpose of this research was to determine the effectiveness of inhibitory of ethanol extract 96% pineapple cobs (Ananas comosus L) to Staphylococcus aureus bacteria growth.  This research is an experimental using post-test design only control group design.  Pineaplle cobs extract 96% by maceration method, Staphycoccus aureus bacteria taken from Mantol salt agar medium.  The results of this study showed the pineapple cobs extrac concentrations of 50% -100% with replication as any 3 replications have an inhibitory to the growth of Staphylococcus aureus bacteria.  Based on One-Way ANOVA test with 95% confidence level there was a significant difference (p <0,05) between various concentrations, so it was continued with LSD test to know the difference in each treatment.  LSD test results showed that 50% concentration was significantly different with concentrations of 70%, 80%, 90% and 100%, while the positive control did not differ significantly with concentrations of 50%.  The conclusion of this research is that pineapple  cobs(Ananas comosus L) has positive effect in inhibitied and killing Staphylococcus aureus bacteria and optimal concentration 70%.  Keywords: pineapple cobs etanol extract, bacitrasin, Staphylococcus aureus, zone of inhibition, antibacterial
乙醇提取物(Ananas Comosus L)对葡萄球菌Aureus的生长的抑制力
非营养素没有得到最佳的利用,而球茎部分含有一些活性成分,其中之一是溴林酶。Ezim bromelin是一种抗菌药物。本研究的目的是研究凤梨酸盐提取物(Ananas comosus L)对葡萄球菌变硬的作用。这项研究是使用post- only control group design设计的实验研究。乙醇提取物(Ananas comosus L)与从介质Manitol salt中提取的葡萄球菌菌的方法额外增加。这项研究发现,菠萝球菌提取物的浓度为50%-100%,三种重复重复,对葡萄球菌aureus的生长有抑制作用。根据95%的信仰水平的单向测试显示,不同浓度之间存在明显的差异(p< 0.05),然后进行LSD测试,以了解不同的治疗方法。LSD测试结果表明,50%的分散与70%、80%、90%和100%的浓度是不同的,而正控制与50%和60%的浓度并没有真正不同。这项研究的结论是,96%的菠萝提取物(Ananas comosus L)对抑制和杀死葡萄球菌aureus和最理想浓度的浓度产生积极影响。关键词:从乙醇提取物中提取96%的菠萝球菌、杆菌、葡萄球菌、肺动脉抑制区目前还没有达到最佳用途,而雄蚕食部分则在此与几种活跃的化合物联系在一起。溴林是一种抗杆菌剂。这项研究的目的是确定乙醇渗出96%的菠萝科对葡萄球菌生长的影响。这个研究是一种实验,使用后的设计只有控制组的设计。马阐明方法,葡萄球菌菌从曼托尔盐中提取一种培养基。这项研究的结果表明,具有50%到100%的菠萝高浓度复制品与3个复制品因葡萄球菌生长而受到抑制。根据95%的定罪率进行的单方面测试,在不同的浓度之间存在着巨大的差异(p < 0.05),所以不断地进行LSD测试,以了解每个治疗的差异。LSD结果表明,50%的集中注意力与70%、80%、90%和100%的集中注意力形成了鲜明的对比,而积极控制并没有显著地影响50%的集中注意力。这项研究的结论是菠萝糖在抑制和杀死葡萄球菌杆菌,最理想的细菌浓度为70%。菠萝科乙醇外露,杆菌,葡萄球菌,抑制区,抗菌
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