Biochemical and Pharmacological Assessments of Tramadol Abuse on Human Male Fertility: Relation to Seminal Plasma 8-Hydroxyguanosine and Zinc.

IF 1.7 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary World Pub Date : 2024-10-01 Epub Date: 2023-06-24 DOI:10.1007/s12291-023-01141-4

Mohammed H Hassan, Aya A Saadeldin, Gamal Alsagheer, Tarek Desoky, Al Shaimaa Hasan

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引用次数: 0

Abstract

Tramadol is a pain killing drug highly used worldwide. There is a knowledge gap for fertility consequences of analgesic addiction in men. In this observational study, we investigated the hazards of tramadol abuse on human male reproductive function. A total of 30 tramadol addicts and 30 healthy controls have participated in the study. History and clinical examination of the included subjects were performed. Biochemical and molecular assays were measured in all participants include serum reproductive hormones (calculated free testosterone, FSH, LH, prolactin and estradiol) using ELISA techniques, semen analysis, seminal plasma zinc and selenium assays using colorimetric kits, seminal plasma tramadol concentrations using Gas Chromatography-Mass Spectrometry (GC-MS), and seminal plasma 8-hydroxyguanosine (8-OHG) using high performance liquid chromatography were measured. Tramadol abuse significantly decreased semen parameters quality. Additionally, tramadol abuse significantly decreased testosterone (P = 0.001) and increased prolactin serum levels (P = 0.000). Tramadol abusers showed significantly higher levels of 8-OHG (P < 0.0001) with significantly lower levels of zinc and selenium in their seminal plasma compared with the controls (P < 0.0001, and 0.0002 respectively). Also, tramadol addicts displayed positive correlations between seminal plasma levels of 8-OHG (r = 0.905, P = 0.00) and sperm abnormal forms (r = 0.610, P = 0.000) with seminal plasma tramadol levels. Seminal plasma levels of zinc (r = - 0.815, P = 0.00), sperm motility (r = - 0.484, P = 0.007), and vitality (r = - 0.430, P = 0.018) were negatively correlated with seminal plasma levels of tramadol. Our data suggest that tramadol abuse may impair male fertility by increasing oxidative damage of sperms and reducing testosterone and the antioxidants trace elements in testicular tissues.

Supplementary information: The online version contains supplementary material available at 10.1007/s12291-023-01141-4.

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滥用曲马多对男性生育能力的生化和药理评估：精浆 8-羟基鸟苷和锌的关系。

曲马多是一种止痛药，在全球范围内被广泛使用。关于镇痛药成瘾对男性生育能力的影响，目前还缺乏相关知识。在这项观察性研究中，我们调查了滥用曲马多对男性生殖功能的危害。共有 30 名曲马多成瘾者和 30 名健康对照者参与了这项研究。我们对研究对象进行了病史和临床检查。所有研究对象都接受了生化和分子检测，包括采用 ELISA 技术检测血清生殖激素（计算游离睾酮、FSH、LH、催乳素和雌二醇）、精液分析、采用比色试剂盒检测精浆锌和硒、采用气相色谱-质谱法（GC-MS）检测精浆曲马多浓度，以及采用高效液相色谱法检测精浆 8-羟基鸟苷（8-OHG）。滥用曲马多会明显降低精液参数质量。此外，滥用曲马多会明显降低睾酮（P = 0.001），增加催乳素血清水平（P = 0.000）。滥用曲马多者的精浆中 8-OHG 水平（P P P = 0.00）和精子异常形态（r = 0.610，P = 0.000）明显高于曲马多水平。精浆锌水平（r = - 0.815，P = 0.00）、精子活力（r = - 0.484，P = 0.007）和精子活力（r = - 0.430，P = 0.018）与精浆曲马多水平呈负相关。我们的数据表明，滥用曲马多可能会增加精子的氧化损伤，减少睾丸组织中的睾酮和抗氧化剂微量元素，从而损害男性生育能力：在线版本包含补充材料，可在 10.1007/s12291-023-01141-4上查阅。

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来源期刊

Veterinary World Multiple-

CiteScore

3.60

自引率

12.50%

发文量

317

审稿时长

16 weeks

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