Induction of Hairy Roots in Cyclobalanopsis glauca and Production of Catechin in Hairy Roots

Q4 Agricultural and Biological Sciences

Taiwan Journal of Forest Science Pub Date : 2012-09-01 DOI:10.7075/TJFS.201209.0239

Minshu Lin, Dacheng Wang

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引用次数: 0

Abstract

Cyclobalanopsis glauca (Thunb.) Oerst is a tree species with rich polyphenol compounds, including catechins, which increase the oxidation resistance of blood plasma and reduce the risk of cardiovascular disease in humans. Catechin may also have an inhibitory effect on carcinogens, specifically those related to skin and lung cancer. This study investigated hairy root induction in leaves of C. glauca infected with 2 strains of Agrobacterium rhizogenes (BCRC15010 and BCRC15785) under the influence of α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). After 45 d, explants co-cultivated with the BCRC15785 strain in MS medium with 0.1 mg L^(-1) NAA and 300 mg L^(-1) cefotaxime exhibited 32.5% greater efficiency of hairy root induction. Induction of calli and hairy roots was observed under the influence of NAA, while only calli were induced under the influence of 2,4-D. We observed the direct influence of the infection period on the transformation frequency, and it increased as the infection period increased up to 24 h. Extracts from hairy roots, calli, and natural seedling roots revealed the presence of catechin content, and concentrations were 4.203, 3.413, and 4.668 mg g^(-1), respectively, in these 3 analytes.

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青冈毛状根的诱导及毛状根中儿茶素的产生

青冈青冈(Cyclobalanopsis glauca)核桃是一种含有丰富多酚化合物的树种，包括儿茶素，可以提高血浆的抗氧化能力，降低人类患心血管疾病的风险。儿茶素也可能对致癌物有抑制作用，特别是那些与皮肤癌和肺癌有关的致癌物。在α-萘乙酸(NAA)和2,4-二氯苯氧乙酸(2,4- d)的作用下，研究了2株发根农杆菌BCRC15010和BCRC15785侵染青桉叶片的毛状根诱导。45 d后，外植体与BCRC15785菌株在MS培养基中共培养，NAA浓度为0.1 mg L^(-1)，头孢噻肟浓度为300 mg L^(-1)，诱导毛状根的效率提高了32.5%。NAA能诱导愈伤组织和毛状根，2,4- d只诱导愈伤组织。我们观察到侵染时间对转化频率的直接影响，随着侵染时间的增加，转化频率逐渐增加，直至24 h。毛状根、愈伤组织和天然幼苗根提取物中均含有儿茶素，浓度分别为4.203、3.413和4.668 mg g^(-1)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Taiwan Journal of Forest Science Agricultural and Biological Sciences-Forestry

CiteScore

0.20

自引率

0.00%

发文量

期刊介绍： The Taiwan Journal of Forest Science is an academic publication that welcomes contributions from around the world. The journal covers all aspects of forest research, both basic and applied, including Forest Biology and Ecology (tree breeding, silviculture, soils, etc.), Forest Management (watershed management, forest pests and diseases, forest fire, wildlife, recreation, etc.), Biotechnology, and Wood Science. Manuscripts acceptable to the journal include (1) research papers, (2) research notes, (3) review articles, and (4) monographs. A research note differs from a research paper in its scope which is less-comprehensive, yet it contains important information. In other words, a research note offers an innovative perspective or new discovery which is worthy of early disclosure.