Analytical Quantification of mycotoxin by LC-ESI-MS/MS, and MALDI-TOF MS in phoma sorghina species of sugarcane

Abstract

Mycotoxin s  are secondary metabolites produced by fungi, which are harmful to humans and animals.  Phoma is a common fungal plant pathogen that can produce mycotoxins in sugarcane. Phoma sorgina  belonging to  phoma  species that cause devastating fungal disease twisted leaf on sugarcane, The present  study  helps  to carry  out research  on potential  metabolites of  fungal  strain belongs to the  phoma sorghina  species such as  P. sorghina  (BS2-1),   P. sorghina  (BS11-1),   P. sorghina   (BSQP)   isolated from sugarcane. These metabolites may influence the functioning of the fungus and are thus important indirectly for its growth and survival.  Mass spectrometry (MS) is commonly used for the detection, characterization and quantitation of mycotoxins in agricultural crops.  Phoma  species were assessed to provide a risk assessment of their secondary metabolites in sugarcane by describing their global metabolome data obtained from the LC-MS instrument. Another study examined through using matrix-assisted laser desorption ionisation – time of flight mass spectrometry (MALDI-TOF MS) to identify  phoma species rapidly and accurately.  Different protein mass spectra obtained with MALDI-TOF MS were able to separate the  phoma  species reported in sugarcane. Therefore, the discovery of new conjugated mycotoxins and secondary metabolites produced  phoma  species was made possible by combining high mass accuracy data with analytic approaches.