Could IL-1β, IL-6, IFN-γ, and sP-selectin serum levels be considered as objective and quantifiable markers of rheumatoid arthritis severity and activity?

IF 1.4 Q3 RHEUMATOLOGY
O. Koper-Lenkiewicz, E. Gińdzieńska-Sieśkiewicz, Joanna Kamińska, A. Milewska, O. Kowal-Bielecka, J. Matowicka-Karna
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引用次数: 0

Abstract

Objectives Rheumatoid arthritis (RA) is a multisystem, chronic, T-cell-mediated disease in which immunological abnormalities result in symmetrical small joint inflammation, articular destruction due to synovitis, and extra-articular organ involvement. An important role in the pathogenesis of RA is attributed to a combination of genetic factors and environmental triggers. Literature data on the utility of circulating IL-1β, IL-6, IFN-γ, and sP-selectin concentration evaluation depending on the activity and advancement of RA seems to be inconclusive. The aim was a case-control study evaluating IL-1β, IL-6, IFN-γ, and sP-selectin concentrations in 77 RA patients dependent on the Steinbrocker classification as well as the disease activity score with examination of 28 joints (DAS28), and compared to 30 control subjects. Material and methods Serum IL-1β, IL-6, IFN-γ, and sP-selectin concentrations were measured using ELISA kits. Results The concentrations of all molecules tested, except for IL-1β, were significantly different from the control group. Univariate logistic regression analysis indicated that their levels significantly influenced the likelihood of RA diagnosis. Differences between IL-1β, IL-6, IFN-γ, and sP-selectin concentrations dependent on the disease activity assessed on the basis of the DAS28 score, as well as the severity of the disease assessed based on the Steinbrocker classification, were not observed. IL-6 positively correlated with the DAS28 score. Conclusions Among the tested molecules, only IL-6 positively correlated with the DAS28 score. Thus, we postulate that next to C-reactive protein and the erythrocyte sedimentation rate, also IL-6 could be clinically relevant and possibly reflects RA activity. Because recently the IL-6 concentration can be determined in applied in vitro diagnostic tests, it presents us with the possibility to test this protein as a marker of RA activity in routine laboratory practice.
IL-1β、IL-6、IFN-γ和sp -选择素的血清水平是否可以作为类风湿关节炎严重程度和活动性的客观和可量化的指标?
类风湿关节炎(RA)是一种多系统、慢性、t细胞介导的疾病,免疫异常导致对称小关节炎症、滑膜炎引起的关节破坏和关节外器官受累。在RA的发病机制中起重要作用的是遗传因素和环境触发因素的结合。关于循环IL-1β, IL-6, IFN-γ和sp -选择素浓度评估依赖于RA的活性和进展的效用的文献数据似乎尚无定论。目的是一项病例对照研究,评估77例RA患者的IL-1β, IL-6, IFN-γ和sp -选择素浓度,依赖于Steinbrocker分类以及28个关节检查的疾病活动评分(DAS28),并与30名对照受试者进行比较。材料与方法采用ELISA试剂盒检测血清IL-1β、IL-6、IFN-γ和sp -选择素浓度。结果除IL-1β外,各组分子浓度均与对照组有显著差异。单因素logistic回归分析表明,它们的水平显著影响RA诊断的可能性。未观察到IL-1β、IL-6、IFN-γ和sp -选择素浓度之间的差异,这些差异取决于基于DAS28评分评估的疾病活动性,以及基于Steinbrocker分类评估的疾病严重程度。IL-6与DAS28评分呈正相关。结论在检测的分子中,只有IL-6与DAS28评分呈正相关。因此,我们假设除了c反应蛋白和红细胞沉降率外,IL-6也可能与临床相关,并可能反映RA的活性。由于最近IL-6的浓度可以在体外诊断试验中测定,这为我们提供了在常规实验室实践中检测该蛋白作为RA活性标记物的可能性。
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来源期刊
Reumatologia
Reumatologia Medicine-Rheumatology
CiteScore
2.70
自引率
0.00%
发文量
44
审稿时长
10 weeks
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