Design of Nanoemulsions Containing Essential Oil of Artemisia Kopetdaghensis: Investigation of Antioxidant Properties and Antimicrobial Activity on Two Isolates from Clinical Specimens
{"title":"Design of Nanoemulsions Containing Essential Oil of Artemisia Kopetdaghensis: Investigation of Antioxidant Properties and Antimicrobial Activity on Two Isolates from Clinical Specimens","authors":"","doi":"10.32592/nkums.15.1.10","DOIUrl":null,"url":null,"abstract":"Introduction: The Artemisia plant is known by its scientific name, which is the exclusive species of Kopeh Daghi in Iran. The present study aimed to design a nanoemulsion of the essential oil of Kopeh Daghi and investigate its antimicrobial activity on the gram-negative bacteria Escherichia coli and the gram-positive bacteria Staphylococcus aureus.\nMethod: At first, the essential oil extraction was performed using a Clevenger. Nanoemulsion was prepared by a spontaneous method using Tween 80 as oil in water. The size of nanoparticles was determined using the dynamic light scattering method. The antioxidant and antibacterial effects of Nanoemulsion at a concentration of 15% of essential oil were investigated.\nResults: In the current research, 38 compounds were identified in the species of Artemisia kopetdaghensis, of which Davanone, with 28.89%, was the most effective substance. The results of the antioxidant test demonstrated that pure essential oil (IC₅₀= 7.05 mg/ml) and Nanoemulsion containing essential oil (IC₅₀= 46.51 mg/ml) had the highest antioxidant activity, respectively. Furthermore, regarding the inhibition of bacterial growth, Nanoemulsion was the most effective in Staphylococcus aureus with a non-growth zone diameter of 8 mm. In Escherichia coli, pure essential oil was the most effective, with a non-growth zone diameter of 9 mm.\nConclusion: In general, the antibacterial properties of 15% nanoemulsion were better compared to 15% essential oil. Moreover, Nanoemulsion containing the medicinal plant essential oil had more antioxidant properties compared to 15% essential oil","PeriodicalId":16423,"journal":{"name":"journal of north khorasan university of medical sciences","volume":"34 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"journal of north khorasan university of medical sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32592/nkums.15.1.10","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
Introduction: The Artemisia plant is known by its scientific name, which is the exclusive species of Kopeh Daghi in Iran. The present study aimed to design a nanoemulsion of the essential oil of Kopeh Daghi and investigate its antimicrobial activity on the gram-negative bacteria Escherichia coli and the gram-positive bacteria Staphylococcus aureus.
Method: At first, the essential oil extraction was performed using a Clevenger. Nanoemulsion was prepared by a spontaneous method using Tween 80 as oil in water. The size of nanoparticles was determined using the dynamic light scattering method. The antioxidant and antibacterial effects of Nanoemulsion at a concentration of 15% of essential oil were investigated.
Results: In the current research, 38 compounds were identified in the species of Artemisia kopetdaghensis, of which Davanone, with 28.89%, was the most effective substance. The results of the antioxidant test demonstrated that pure essential oil (IC₅₀= 7.05 mg/ml) and Nanoemulsion containing essential oil (IC₅₀= 46.51 mg/ml) had the highest antioxidant activity, respectively. Furthermore, regarding the inhibition of bacterial growth, Nanoemulsion was the most effective in Staphylococcus aureus with a non-growth zone diameter of 8 mm. In Escherichia coli, pure essential oil was the most effective, with a non-growth zone diameter of 9 mm.
Conclusion: In general, the antibacterial properties of 15% nanoemulsion were better compared to 15% essential oil. Moreover, Nanoemulsion containing the medicinal plant essential oil had more antioxidant properties compared to 15% essential oil