Acquisition of plastid movement responsiveness to light during mesophyll cell differentiation.

Joanna Augustynowicz, Weronika Krzeszowiec, Halina Gabrys
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引用次数: 6

Abstract

A culture of Nicotiana tabacum leaf protoplasts, regenerating in vitro, was used to study light-induced plastid translocations. Experiments were carried out for 5 months starting with protoplasts, through single cells, microcolonies and callus to the differentiated mesophyll of regenerated plants. Although the actin cytoskeleton was fully developed at every stage of culture, blue light-mediated directional movements of chloroplasts were observed only after the full differentiation of callus into leaf tissues. These chloroplast rearrangements were similar to those observed in control plants grown from seeds. Under strong blue light, chloroplasts gathered at the cell walls parallel to the light direction (profile position); under weak blue light, they gathered at the walls perpendicular to the light direction (face position). No light-dependent plastid arrangements were found in undifferentiated cell cultures even after cell wall recovery. A characteristic pattern of plastids in the dividing cells was independent of light signals. Only trace chloroplast responses to strong blue light were detected in the first leaves regenerating from callus. We hypothesize that factors which control the developmental status of a cell, i.e. division and differentiation, take over the control of plastid redistribution from light signals.

在叶肉细胞分化过程中获得质体运动对光的反应能力。
研究人员利用体外再生的烟草叶片原生质体培养物来研究光诱导的质体易位。实验从原生质体开始,经过单细胞、小细胞群、胼胝体到再生植株的分化叶肉,历时 5 个月。虽然肌动蛋白细胞骨架在培养的每个阶段都得到了充分发展,但只有在胼胝体完全分化成叶组织后,才能观察到蓝光介导的叶绿体定向移动。这些叶绿体的重新排列与从种子培育的对照植物中观察到的叶绿体重新排列相似。在强蓝光下,叶绿体聚集在平行于光照方向的细胞壁上(剖面位置);在弱蓝光下,叶绿体聚集在垂直于光照方向的细胞壁上(面位置)。在未分化的细胞培养物中,即使细胞壁恢复后,也没有发现与光有关的质体排列。分裂细胞中质体的特征模式与光信号无关。在从胼胝体再生的第一片叶子中,只检测到叶绿体对强蓝光的微量反应。我们假设,控制细胞发育状态的因素,即分裂和分化,取代了光信号对质体再分布的控制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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