In-vitro Antioxidant, Antibacterial and Phytochemical Properties of Various Solvents Extracts of Eclipta alba Against Isolated ESBL Uropathogens

Abstract

The main source of food, shelter, and many therapeutic techniques is provided by plants. Since ancient times, they have been used to treat a variety of human illnesses all throughout the world. Our study focused on assessing the antimicrobial activities and antioxidant activities of Eclipta.alba (prostrata) crude extracts (whole plant) using the in-vitro method. Different polarity-based solvent extracts of Eclipta.alba (prostrate) were screened against ESBL producing pathogens such as Esherichia coli, klebsiella pneumoniae, Enterobacter aerogens, Proteus mirabilis, and Pseudomonas aeruginosa. Out of all the tested extracts, aqueous extracts of E. alba exhibited the most effective activity against K. pneumoniae (18±0.577). Results obtained from all the extracts were compared with standard antibiotic amikacin. The antioxidant activity was determined using a DPPH assay and the data obtained showed that extracts of E. alba (prostrate) were highly antioxidant. GC-MS and HPLC analysis revealed the presence of significant bioactive compound. The reference compound used to compare the results of antioxidant values was Gallic acid. The study provides valuable evidence of the antibacterial activity of of E.alba (prostrata) crude extracts (Whole plant) in curing Urinary Tract Infections.