Biomimetic scaffold development for bone tissue engineering: Crosslinking graphene with collagen to enhance mechanical strength, conductivity, and porous structure

IF 0.7 4区材料科学 Q4 METALLURGY & METALLURGICAL ENGINEERING

International Journal of Materials Research Pub Date : 2023-08-29 DOI:10.1557/s43578-023-01145-z

P. Rebecca, D. Durgalakshmi, S. Balakumar, R. A. Rakkesh

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引用次数: 0

Abstract

In this study, we extract type I collagen from fish scales and employ an electrostatic self-assembly technique to crosslink it with negatively charged graphene. By incorporating 0%, 1%, 5%, and 10% weight of graphene with collagen, we significantly enhance the mechanical strength, conductivity, and 3D porous structure of the scaffolds. The incorporation of graphene increases the Young’s modulus of the scaffolds threefold compared to pure collagen scaffolds. Impedance measurements reveal values of 4 kΩ, 2.5 kΩ, and 1 kΩ for scaffolds containing 1%, 5%, and 10% weight of graphene with collagen, respectively. The scaffolds demonstrate cell viability above 90%, and the osteogenic differentiation potential, as determined by ALP assay, confirms successful osteogenesis. Moreover, the eco-friendly synthesis route establishes the hybrid 3D graphene-collagen nanocomposite scaffold as a stable material with excellent biocompatible properties in a biological medium.

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骨组织工程仿生支架开发:石墨烯与胶原交联增强机械强度、导电性和多孔结构

在这项研究中，我们从鱼鳞中提取I型胶原蛋白，并采用静电自组装技术将其与带负电荷的石墨烯交联。通过将0%、1%、5%和10%重量的石墨烯掺入胶原蛋白中，我们显著提高了支架的机械强度、导电性和3D多孔结构。与纯胶原蛋白支架相比，石墨烯的掺入使支架的杨氏模量增加了三倍。阻抗测量显示，含有1%、5%和10%石墨烯和胶原的支架分别为4 kΩ、2.5 kΩ和1 kΩ。支架的细胞存活率在90%以上，ALP测定的成骨分化潜力证实成骨成功。此外，生态友好的合成路线建立了3D石墨烯-胶原纳米复合支架作为一种稳定的材料，在生物介质中具有优异的生物相容性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Materials Research 工程技术-冶金工程

CiteScore

1.30

自引率

12.50%

发文量

119

审稿时长

6.4 months

期刊介绍： The International Journal of Materials Research (IJMR) publishes original high quality experimental and theoretical papers and reviews on basic and applied research in the field of materials science and engineering, with focus on synthesis, processing, constitution, and properties of all classes of materials. Particular emphasis is placed on microstructural design, phase relations, computational thermodynamics, and kinetics at the nano to macro scale. Contributions may also focus on progress in advanced characterization techniques. All articles are subject to thorough, independent peer review.