Identification and characterization of novel short-type BmPGRP-S4 from the silkworm, Bombyx mori, involved in innate immunity

Qiang Wang, Meijia Ren, Xiaoyong Liu, H. Xia, Keping Chen
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引用次数: 6

Abstract

Abstract Peptidoglycan recognition proteins (PGRPs) are pattern recognition receptors that can recognize bacterial peptidoglycans and trigger the innate immune response of insects. Here, we identified and characterized a novel short-type Bombyx mori peptidoglycan recognition proteins short-4 (BmPGRP-S4) in a lepidopteran insect, Bombyx mori. BmPGRP-S4 exhibited a cDNA sequence length of 600 bp, encoding 199 aa with a protein molecular weight of 22 kDa. Multiple sequence alignment revealed that BmPGRP-S4 contains a conserved PGRP domain. Quantitative real-time polymerase chain reaction analysis showed that BmPGRP-S4 is highly expressed in the early developmental stages of silkworm larvae and presents tissue-specific expression in hemocytes. Interestingly, BmPGRP-S4 expression is significantly induced by bacterial infection in the midgut, fat body, and hemocytes. Furthermore, a dual luciferase reporter gene assay revealed that BmPGRP-S4 can activate the expression of the antimicrobial peptide genes lebocin, moricin, cecropin D, cecropin B, and attacin. Taken together, these results suggest that BmPGRP-S4 plays an important role in the innate immune response of silkworms.
家蚕先天免疫相关基因新型短型BmPGRP-S4的鉴定与表征
肽聚糖识别蛋白(PGRPs)是一种模式识别受体,可以识别细菌肽聚糖并引发昆虫的先天免疫反应。本研究在鳞翅目昆虫家蚕中鉴定并鉴定了一种新的短型家蚕肽聚糖识别蛋白short-4 (BmPGRP-S4)。BmPGRP-S4 cDNA序列长度为600 bp,编码199 aa,蛋白分子量为22 kDa。多个序列比对结果表明,BmPGRP-S4含有一个保守的PGRP结构域。定量实时聚合酶链反应分析显示,BmPGRP-S4在家蚕幼虫发育早期高表达,并在血细胞中呈组织特异性表达。有趣的是,细菌感染显著诱导BmPGRP-S4在中肠、脂肪体和血细胞中的表达。此外,双荧光素酶报告基因检测显示,BmPGRP-S4可以激活抗菌肽基因lebocin、moricin、cecropin D、cecropin B和attacin的表达。综上所述,这些结果表明BmPGRP-S4在家蚕先天免疫应答中起重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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