Penetration of Dialysis Tubes to Components Absorbed in the Plasma of the Extract of Fructus Evodiae: Methodology of the Preparation of Herbal Extract for Pharmacological Experiment in Vitro

World Science and Technology Pub Date : 2010-08-01 DOI:10.1016/S1876-3553(11)60022-7

Li Huiying , Wang Xiukun , Wang Yugang , Zhan Honglei , Hu Jun , Hua Lei , Lei Fan , Xing Dongming , Wang Zhimin , Du Lijun

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Abstract

Dialysis tubes (Mw 100 D and Mw 500 D) were used to dialyze and purify the water extraction of Wuzhuyu (Fructus Evodiae) and Wuzhuyu decoction, and three parts of molecular weight less than 100 D, between 100 and 500 D, and more than 500 D were obtained. To ascertain the peak time point efficiency, several chemicals treated at different time periods by high performance liquid chromatography (HPLC) were analyzed, and the activity of blood vessels was studied in vitro. By comparing different dialyzing times for three chemicals including evodiamine, isorhamnetin, and limonin in 100–500 D, the best treatment time was found to be 48 h. The portion more than 100 D significantly contracted blood vessels, while the portion less than 100 D did not. In conclusion, it may be feasible to dispose samples using dialysis tubes with different apertures and apply the extract to pharmacological evaluation in vitro.

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透析管对枸杞提取物血浆中吸收成分的渗透作用:体外药理实验用中药提取物的制备方法

采用透析管(Mw 100d和Mw 500d)对乌茱萸水提物和乌茱萸汤进行透析纯化，得到了分子量小于100d、100 ~ 500d和大于500d的三种组分。为确定峰值时间点效率，采用高效液相色谱法(HPLC)对不同时间处理的几种化学药品进行了分析，并对其体外血管活性进行了研究。通过对evolodiamine、异鼠李素和柠檬苦素3种化学物质在100 - 500 D条件下不同透析时间的比较，发现最佳的处理时间为48 h。超过100 D的部分血管收缩明显，而小于100 D的部分血管收缩不明显。综上所述，采用不同孔径的透析管处理样品，并将其用于体外药理评价是可行的。

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World Science and Technology

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