Pendant liposome system to access the internal solution

Abstract

This paper aimed to develop a pendant liposome system that allows the access to both internal and external solutions of the formed liposomes. The access to the internal solution was achieved by a through-hole fabricated on the substrate, which was designed underneath the pendant-like, dome-shaped liposome. The formation of a uniform-size liposome array was based on our previous works that applied an electrospray deposition technique for a lipid micropatterning. With rehydration of the lipid pattern, we could observe a formation of a dome-shaped liposome array covering over the holes and a release of the liposomes. We further intend for exchanging the internal solution and/or encapsulating biological components with the developed system.