The response of lucerne and red clover roots to aluminium/hematoxylin: how universal is the hematoxylin test for aluminium?

R. Bennet
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引用次数: 6

Abstract

Despite the long association between hematoxylin and aluminium (Al) in studies of Al rhizotoxicity, it is evident that many aspects of the hematoxylin test are not fully understood. Controlled environment studies of the responses of lucerne and red clover roots to the Al/hematoxylin system have indicated that the intensity and distribution of the stain in root tissue was influenced by, (1) the pH of the growing medium, and (2) the age of the root at the time of the treatment. In the younger (≦ 120 h), Al-treated (30 μM; 24 h) roots the highest intensity of hematoxylin stain was encountered at pH 4.1. In contrast, some roots (pH 4.5 ≦ 72 h) did not respond at all to hematoxylin, although growth studies indicated that a physiologically active Al component was present in these roots. Intermediate pH's (4.3) resulted in intermediate stain intensities and a spiral motif of root stain was present in many of these roots (72 h and 120 h). The roots' affinity for hematoxylin increased with age. At 192 h, the pH-de...
紫花苜蓿和红三叶草根对铝/苏木精的反应:苏木精对铝的检测有多普遍?
尽管苏木精和铝(Al)在铝根毒性研究中的长期关联,但很明显苏木精试验的许多方面尚未完全了解。苜蓿和红三叶草根系对Al/苏木精系统反应的受控环境研究表明,根系组织中染色的强度和分布受以下因素的影响:(1)生长培养基的pH值;(2)处理时根系的年龄。较年轻(≦120 h), al处理(30 μM;24 h时,pH为4.1时苏木精染色强度最高。相比之下,有些根系(pH值4.5≦72 h)对苏木精完全没有反应,尽管生长研究表明这些根系中存在生理活性的Al成分。中等pH值(4.3)导致中等染色强度,并且在许多根(72 h和120 h)中存在根染色的螺旋基序。根对苏木精的亲和力随着年龄的增长而增加。在192 h时,pH-de。
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