In vitro and in vivo evaluation of porous alginate hydrogel containing retinoic acid for skin wound healing applications

IF 2.1 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Bioactive and Compatible Polymers Pub Date : 2022-06-07 DOI:10.1177/08839115221104071

Simin Nazarnezhad, M. Salehi, H. Samadian, Arian Ehtermi, N. Kasaiyan, H. Khastar, Ghasem Abbaszadeh-Goudarzi, Nariman Rezaei Kolarijani, Hodays Yeganehfard, H. Ziaei

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引用次数: 1

Abstract

The current study’s main aim was to fabricate and evaluate alginate (Alg) hydrogel containing retinoic acid (RA) as wound healing materials. Different RA concentrations (2, 10, and 50% w/w) were incorporated into the hydrogel. The results showed that the prepared hydrogels had a porous structure with a pore size of 69.69 ± 22.1 µm for pure Alg hydrogel and 78.44 ± 27.8 µm for Alg/RA hydrogel. The swelling measurement showed that the hydrogels swelled up to 65% and the incorporation of RA reduced the degree of swelling . The in vitro studies confirmed the hemo- and biocompatibility of the Alg/RA 2% and increasing the RA concentration induced hemolysis and toxic effects. The animal studies showed that the lowest RA concentration resulted in the best treatment outcome while increasing the RA concentration suppressed the healing process. In conclusion, these results showed that RA induced wound healing process at low concentration, and the prepared hydrogel could be used as the wound healing materials.

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含维甲酸的多孔海藻酸盐水凝胶用于皮肤伤口愈合的体外和体内评价

本研究的主要目的是制备和评价含有维甲酸(RA)的海藻酸盐水凝胶作为伤口愈合材料。水凝胶中加入不同浓度的RA(2、10和50% w/w)。结果表明，制备的水凝胶具有多孔结构，纯Alg水凝胶的孔径为69.69±22.1µm, Alg/RA水凝胶的孔径为78.44±27.8µm。溶胀测量结果表明，水凝胶溶胀达65%，RA的加入降低了溶胀程度。体外研究证实了Alg/RA 2%的血液和生物相容性，并增加RA浓度诱导溶血和毒性作用。动物实验表明，RA浓度最低时治疗效果最好，而RA浓度升高会抑制愈合过程。综上所述，这些结果表明，低浓度的RA诱导创面愈合，制备的水凝胶可以作为创面愈合材料。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Bioactive and Compatible Polymers 工程技术-材料科学：生物材料

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： The use and importance of biomedical polymers, especially in pharmacology, is growing rapidly. The Journal of Bioactive and Compatible Polymers is a fully peer-reviewed scholarly journal that provides biomedical polymer scientists and researchers with new information on important advances in this field. Examples of specific areas of interest to the journal include: polymeric drugs and drug design; polymeric functionalization and structures related to biological activity or compatibility; natural polymer modification to achieve specific biological activity or compatibility; enzyme modelling by polymers; membranes for biological use; liposome stabilization and cell modeling. This journal is a member of the Committee on Publication Ethics (COPE).