Evaluation of an Automatic Device to Prepare Fibronectin Eyedrops Using a Patient's Own Plasma.

Abstract

Fibronectin (FN) has been reported to be effective for the treatment of corneal trophic ulcers, but the FN eyedrop preparation is not a simple technique. We previously developed an automatic device to prepare FN eyedrops using a patient's own plasma (Yoshida et al. Jpn. J. Hosp. Pharm., 24, 493-498 (1998)). The aim of this study was to evaluate the efficiency of this automatic device. FN eyedrops were prepared using plasma from healthy individuals either with an automatic device or a manual process using gelatin-coupled Sepharose 4 B (GS 4 B) and Sephadex G-25 (PD-10), The average FN concentration and the recovery rate in the eyedrops