Effect of Eight Weeks of Interval Training on Amyloid Precursor Protein (APP) Gene Expression in Hippocampal Tissue in Methamphetamine-dependent Rats (Crystal)

{"title":"Effect of Eight Weeks of Interval Training on Amyloid Precursor Protein (APP) Gene Expression in Hippocampal Tissue in Methamphetamine-dependent Rats (Crystal)","authors":"","doi":"10.32592/nkums.15.1.79","DOIUrl":null,"url":null,"abstract":"Introduction: Methamphetamine (METH) is a stimulant drug that causes memory and learning disorders. The present study aimed to investigate the effect of eight weeks of interval training on APP gene expression in hippocampal tissue and open field test in methamphetamine-dependent rats.\nMethod: In this experimental research, 32 rats were assigned to four equal groups of saline, primary methamphetamine (METH-1), methamphetamine+training (METH+MIT), and secondary methamphetamine (METH-2). The METH was injected at the dose of 5 mg/kg body weight for 21 days. The exercise program (5 sessions per week) included interval training (4 sets of 4 minutes with moderate intensity and 2 minutes of active rest between sets) on a treadmill. At the end of the injection and training period, the hippocampus tissue of the rats was extracted to evaluate gene expression changes. An open-field behavioral test was also conducted. The data were analyzed using the one-way ANOVA at the significance level of P≥0.05.\nResults: Methamphetamine significantly increased APP gene expression in METH-1,2 groups compared to saline (respectively; P≥0.007 and P≥0.005). The total distance and movement speed of rats in METH-1 and 2 groups had a significant decrease compared to saline (respectively, P≥0.004 and P≥0.026). The MIT led to a significant reduction of APP compared to METH-1,2 groups (respectively, P≥0.041 and P≥0.028). Movement speed in the MIT group increased significantly compared to the METH-1 group (P≥0.008).\nConclusion: Methamphetamine reduced cognitive performance (learning and memory), and according to the results, MIT probably improved cognitive performance. Nonetheless, further studies are necessary to reach definitive conclusions.","PeriodicalId":16423,"journal":{"name":"journal of north khorasan university of medical sciences","volume":"4 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"journal of north khorasan university of medical sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32592/nkums.15.1.79","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Introduction: Methamphetamine (METH) is a stimulant drug that causes memory and learning disorders. The present study aimed to investigate the effect of eight weeks of interval training on APP gene expression in hippocampal tissue and open field test in methamphetamine-dependent rats. Method: In this experimental research, 32 rats were assigned to four equal groups of saline, primary methamphetamine (METH-1), methamphetamine+training (METH+MIT), and secondary methamphetamine (METH-2). The METH was injected at the dose of 5 mg/kg body weight for 21 days. The exercise program (5 sessions per week) included interval training (4 sets of 4 minutes with moderate intensity and 2 minutes of active rest between sets) on a treadmill. At the end of the injection and training period, the hippocampus tissue of the rats was extracted to evaluate gene expression changes. An open-field behavioral test was also conducted. The data were analyzed using the one-way ANOVA at the significance level of P≥0.05. Results: Methamphetamine significantly increased APP gene expression in METH-1,2 groups compared to saline (respectively; P≥0.007 and P≥0.005). The total distance and movement speed of rats in METH-1 and 2 groups had a significant decrease compared to saline (respectively, P≥0.004 and P≥0.026). The MIT led to a significant reduction of APP compared to METH-1,2 groups (respectively, P≥0.041 and P≥0.028). Movement speed in the MIT group increased significantly compared to the METH-1 group (P≥0.008). Conclusion: Methamphetamine reduced cognitive performance (learning and memory), and according to the results, MIT probably improved cognitive performance. Nonetheless, further studies are necessary to reach definitive conclusions.
8周间歇训练对甲基苯丙胺依赖大鼠海马组织淀粉样前体蛋白(APP)基因表达的影响(晶体)
简介:甲基苯丙胺(冰毒)是一种兴奋剂,会导致记忆和学习障碍。本研究旨在探讨8周间歇训练对甲基苯丙胺依赖大鼠海马组织APP基因表达和开放场试验的影响。方法:将32只大鼠随机分为生理盐水、甲基苯丙胺(METH-1)、甲基苯丙胺+训练(METH+MIT)和甲基苯丙胺(METH-2) 4组。注射剂量为5 mg/kg体重,连续注射21天。锻炼计划(每周5次)包括在跑步机上进行间歇训练(4组,每组4分钟,强度适中,每组之间休息2分钟)。在注射和训练结束时,提取大鼠海马组织,评估基因表达变化。还进行了野外行为测试。数据分析采用单因素方差分析,显著性水平P≥0.05。结果:甲基苯丙胺与生理盐水(分别;P≥0.007和P≥0.005)。与生理盐水相比,METH-1、meth - 2组大鼠总距离和运动速度显著降低(P≥0.004、P≥0.026)。与METH-1、2组相比,MIT导致APP显著降低(P分别≥0.041和P≥0.028)。与METH-1组相比,MIT组的运动速度明显增加(P≥0.008)。结论:甲基苯丙胺降低了认知表现(学习和记忆),根据结果,MIT可能改善了认知表现。尽管如此,需要进一步的研究才能得出明确的结论。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信