Validation of an Et-Aas Method for the Determination of Pb and Cd in Liver and Kidney Samples of Brown Hare (Lepus Europaeus P.)

Abstract

A rapid, sensitive, accurate and precise method for the determination of lead and cadmium in tissue samples of hare by Electro-thermal Atomic Absorption Spectrometry was validated. The main parameters evaluated in the validation were: linearity, accuracy, repeatability and within-laboratory reproducibility, detection and quantification limits. The results for limit of detection and limit of quantification were 4.26 and 12.90 µg/kg for lead and for cadmium were 2.32 and 7.04 µg/kg. Method showed good linearity R2=0,997 for cadmium and R2=0,999 for lead. Accuracy was evaluated by spike recovery tests and it was performed for lead and cadmium in both, kidney and liver samples. Obtained recovery values were ranged from 81.6% to 107.0%. Overall measure uncertainties were 33% for lead and 29% for cadmium. The results make evident that the procedure can be used as a method for the determination of lead and cadmium in liver and kidney samples of brown hare.