Teratomas produced from human pluripotent stem cells xenografted into immunodeficient mice - a histopathology atlas.

Ivan Damjanov, Peter W Andrews
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Abstract

This atlas illustrates the microscopic features of tumors produced from human pluripotent stem cells (hPSCs) xenografted into immunosuppressed mice, according to the generally accepted protocols for performing this teratoma assay of stem cell pluripotency. Microphotographs depict various hematoxylin and eosin (H&E) stained tissues derived from all three embryonic germ layers (ectoderm, mesoderm and endoderm). The appearance of persistent hPSC in teratomas is also described with special emphasis on the morphogenesis of embryoid bodies and yolk sac components surrounding them. The use of immunohistochemistry for analyzing hPSC-derived teratomas is also illustrated.

将人多能干细胞异种移植到免疫缺陷小鼠体内产生的畸胎瘤--组织病理学图谱。
本图集展示了人多能干细胞(hPSCs)异种移植到免疫抑制小鼠体内产生的肿瘤的显微特征,这些肿瘤是根据公认的干细胞多能性畸胎瘤检测方案产生的。显微照片描绘了来自所有三个胚胎胚层(外胚层、中胚层和内胚层)的各种苏木精和伊红(H&E)染色组织。此外,还描述了畸胎瘤中持续存在的 hPSC 的外观,特别强调了类胚体及其周围卵黄囊成分的形态发生。此外,还说明了如何使用免疫组化技术分析源自 hPSC 的畸胎瘤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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