The Cytotoxic Activities of the Extracts of Sengkubak (Pycnarrhena cauliflora) As Apoptosis Inducers to Hela Cervical Cancer Cells

Masriani
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引用次数: 3

Abstract

Cancer has been considered as problematic medical issues both in developed and developing countries. Pycnarrhena cauliflora or Sengkubak (Pycnarrhena cauliflora) has a potential value as alternatives for anticancer medication. This study aims to evaluate the cytotoxic activities of Sengkubak (Pycnarrhena cauliflora), and investigate the induction for apoptosis mechanisms from the extracts n-hexane, dichloromethane, and methanol from roots, branches the leaves to HeLa cancer cells. The cytotoxic activities were analyzed by performing MTT methods, while induction analysis of apoptosis was conducted by utilizing the Acridine Orange/Ethidium Bromide (AO/EB) as visual nuclear methodologies. The extracts of roots dichloromethane showed the highest cytotoxic activities accounted for IC50 70µg/mL and these extracts were able to induce the apoptosis mechanisms. This study concludes that the Sengkubak (Pycnarrhena cauliflora) has the ability as a potential alternative for anticancer medications.
仙姑草提取物对Hela宫颈癌细胞的细胞毒活性及其诱导凋亡作用
无论是在发达国家还是在发展中国家,癌症都被视为有问题的医疗问题。花椰菜或仙库柏(花椰菜)作为抗癌药物的替代品具有潜在的价值。本研究旨在评价山毒草(pycnarhena cauliflora)的细胞毒活性,探讨其根、枝、叶中正己烷、二氯甲烷和甲醇提取物诱导HeLa癌细胞凋亡的机制。采用MTT法分析其细胞毒活性,以吖啶橙/溴化乙啶(AO/EB)为目视核方法进行细胞凋亡诱导分析。根二氯甲烷提取物的细胞毒活性最高,为IC50 ~ 70µg/mL,具有诱导细胞凋亡的机制。本研究认为,生库巴克(pycnarhena cauliflora)具有潜在的抗癌药物替代能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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