Identifying the conditions necessary for the thioredoxin ultrasensitive response

Perspectives in science Pub Date : 2016-12-01 DOI:10.1016/j.pisc.2016.05.011

Johann M. Rohwer , Charl Viljoen , Carl D. Christensen , Lefentse N. Mashamaite , Ché S. Pillay

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引用次数: 8

Abstract

Thioredoxin, glutaredoxin, and peroxiredoxin systems (collectively called redoxins) play critical roles in a large number of redox-sensitive cellular processes. These systems are linked to each other by coupled redox cycles and by common reaction intermediates into a larger network.

Previous results from a realistic computational model of the Escherichia coli thioredoxin system developed in our group have revealed several modes of kinetic regulation in the system. Amongst others, the coupling of the thioredoxin and peroxiredoxin redox cycles was shown to exhibit the potential for ultrasensitive changes in the thioredoxin concentration and the flux through other thioredoxin-dependent processes in response to changes in the thioredoxin reductase level. Here, we analyse the basis for this ultrasensitive response using kinetic modelling and metabolic control analysis and derive quantitative conditions that must be fulfilled for ultrasensitivity to occur.

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确定硫氧还蛋白超敏反应的必要条件

硫氧还蛋白、戊二氧还蛋白和过氧化物还蛋白系统(统称为氧化还蛋白)在大量氧化还原敏感的细胞过程中起着关键作用。这些系统通过耦合的氧化还原循环和共同的反应中间体相互连接成一个更大的网络。我们小组开发的大肠杆菌硫氧还蛋白系统的实际计算模型的先前结果揭示了该系统中的几种动力学调节模式。除其他外，硫氧还蛋白和过氧还蛋白氧化还原循环的偶联显示出硫氧还蛋白浓度和其他硫氧还蛋白依赖过程的通量的超敏感变化的潜力，以响应硫氧还蛋白还原酶水平的变化。在这里，我们使用动力学建模和代谢控制分析来分析这种超灵敏反应的基础，并推导出超灵敏发生必须满足的定量条件。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Perspectives in science

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