"Rapid and Practical Screening Method for the Detection of Colistin-Resistant Bacteria in Food"

Abstract

Rapid and Practical Screening Method for the Detection of Colistin-Resistant Bacteria in Food. Biomed J Sci & Res We developed a rapid and practical screening method for simultaneously detecting both Escherichia coli and bacteria harboring the mcr-1 colistin resistance gene using high-speed real-time polymerase chain reaction with specific TaqMan probes. The entire procedure, from sample processing to the final result, was performed within 1 h. The practical utility of this method was verified by analyzing 27 retail meat samples for the presence of colistin-resistant bacteria. The results indicated the potential of this method for the convenient and rapid screening of food items to detect contamination with mcr-1 –positive bacteria, which can be especially useful for on-site testing in developing countries.