Production and Some Properties of Branched Cyclodextrins

Abstract

Cyclodextrins (CDs, tt cyclomaltooligosaccharides) with maltosyl and panosyl branches were produced from maltose or panose and CDs by the reverse action of pullulanase. Purification on columns of octadesylated silica gave G2-α-CD, G2-β-CD, Pan-α-CD, and Pan-β-CD. The solubility of G2-β-CD in aqueous 80% ethanol was higher than that of β-CD in water. The relative rates of degradation of maltose, G2-α-CD, and G2-β-CD with glucoamylase were 1 : 3.6 : 5.0 and those of panose, Pan-α-CD, and Pan-β-CD were 1 : 3.0 : 2.2. The rates of degradation of branched and unbranched maltooligosaccharides were markedly different from those of G2-CDs and Pan-CDs.