A self-assembly conductive device for direct DNA identification in integrated microarray based system

Abstract

A self-assembly conductive method for DNA identification has been demonstrated to be used in an array based microelectronic biochip. The detailed fabrication steps of the device and the procedures to identify targeted samples with gold nanoparticle labels are presented. By using the pre-hybridization and amplification method, the sensitivity of this array-based microelectronic biochip exceeds that of a traditional fluorescence detection system by three orders of magnitude. The device is simple and compatible with standard IC fabrication technology with slight modification to the last passivation and metallization process. A substantial difference in current is measured from the device with matched and unmatched DNA samples, which can be easily detected by any modern IC comparator.