BT-474 Breast Cancer Cell Apoptosis Induced by Crocin, a Saffron Carotenoid

Nassim Faridi, H. Heidarzadeh, M. Mohagheghi, S. Z. Bathaie
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引用次数: 8

Abstract

Background: Saffron carotenoids have been known as powerful phytochemicals in breast cancer treatment. The purpose of this study was to investigate the anti-cancer properties of an important saffron carotenoid, crocin, on BT-474 which is a known HER2+ breast cancer cell line. Methods: The effect of crocin on cell viability was investigated using MTT assay. Apoptosis induction was studied via flow cytometry and Western blotting of caspase-9 and cleaved caspase-9. Involvement of unfolded protein response (UPR) was also investigated via RT-PCR study of the XBP1 gene. Results: The results showed that crocin treatment decreases the viability of BT-474 cells and induces early and late apoptosis in these cells. The mechanism of crocin action was through the induction of caspase-9 expression and cleavage. Furthermore, we found that crocin induced XBP1 gene splicing in these cells. Conclusion: The present study provides important evidence that crocin induces apoptosis in BT-474 cells. In addition, the activation of UPR may play a role in the anticancer effects of crocin.
藏红花类胡萝卜素藏红花素诱导BT-474乳腺癌细胞凋亡
背景:藏红花类胡萝卜素被认为是治疗乳腺癌的有效植物化学物质。本研究的目的是研究一种重要的藏红花类胡萝卜素藏红花素对已知HER2+乳腺癌细胞系BT-474的抗癌特性。方法:采用MTT法研究藏红花素对细胞活力的影响。通过流式细胞术和Western blot检测caspase-9和cleaved - caspase-9对细胞凋亡的诱导作用。通过RT-PCR研究XBP1基因的未折叠蛋白反应(UPR)参与情况。结果:藏红花素可降低BT-474细胞活力,诱导早期和晚期细胞凋亡。藏红花素的作用机制是通过诱导caspase-9的表达和裂解。此外,我们发现藏红花素在这些细胞中诱导XBP1基因剪接。结论:本研究为藏红花素诱导BT-474细胞凋亡提供了重要证据。此外,UPR的激活可能在藏红花素的抗癌作用中发挥作用。
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