Comparative evaluation of the biological activity of a complex of ultrafine particles based on copper and zinc in the bioluminescence inhibition test

Abstract

Modern methods of nanotechnology make it possible to transform macro- and micro-objects into ultrafine particles (UFP), while the process itself is accompanied by a change in the chemical and physical properties of the base material. Naturally, the biological activity of complexes of UHF microelements will differ from that of individual UHFs. In this regard, the purpose of our study was to study the dependence of the biological activity of the UHF copper + zinc complex on their physicochemical characteristics.Analysis of the data obtained allowed us to state that sample «C» had a high level of toxicity of the studied samples of UHF against the strain E. coli E. coli K12 TG1 pF1. Further, according to the observed effect, sample A was noted, while the effect of exposure to the studied UHF was dose- and time-dependent.As a result, the data obtained on the basis of the reaction of strains E. coli pSoxS::lux and E. coli pKatG-lux, the induction of luminescence of which is associated with the development of oxidative stress in cells, on the action of the studied samples of the copper+ zinc complex, made it possible to form a sample with the maximum effect «C». Further, according to the degree of development of the toxic effect, samples «B» and «A» decreased in the series. At the same time, the interpretation of the results of the response of the E. coli pRecA -lux strain, the induction of which is caused by DNA damage in cells, forms a different series of specificity: the greatest effect on bacterial cells among the studied substances, sample B was rendered, while the maximum luminescence amplitude was 70 units at 0.002 M, and the sensitivity threshold was at a concentration of 0.0004 M.Based on the data obtained, in our study, the degree of biological activity of UHF for a living system was determined by a combination of a number of physicochemical parameters: shape, phase composition, and specific surface area