Genetic diversity assessment of Aegilops germplasm by RAPD molecular markers

Abstract

Study of the genetic diversity in plant inherited stores in order to classify the germplasm regarding resistance to biotic and biotic stress and also preventing from genetic erosion is one of the basic and fundamental steps in the most breeding programs .Iran is one of the most important centers of genetic diversity due to having different climates and the old civilization. In order to distinguish and evaluate different genetic of aegilops germplasm, which were collected fromwest part of the Iran, 80 ecotypes of aegilops by RAPDmolecularmarkerwere assessed .DNAextractionwas done bymodified CTAB method.After DNAextraction stages, complement gene locuses were amplified by 18 RAPD primers. These primers produced 183 bands, that 151 bands (about 82%) were polymorphic. Cluster analysis based on the resulting datawas performed usingUPGMAmethod and DiceÂÂ’s similarity coefficient in NTSYS software. The resulting dendogram categorized the accessions into 8 groups in 69%similarity. Principle ComponentAnalysis (PCA) was performed too, 2 and 3 dimensions graphs using 18 primers were drawn.