Aqueous extract OF Moringa oleifera Lam leaves added to freezing extenders damages goat sperm membranes.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-05-01 DOI:10.54680/fr23310110712

Desirée Coelho de Mello Seal, M. M. Monteiro, L. Arruda, Jerônimo Hugo de Souza, Robson Raion de Vasconcelos Alves, Luiz Alberto Lira Soares, T. Napoleão, P. M. Guedes Paiva, Lucas Eduardo Bezerra de Lima, Regina C. Bressan Queiroz de Figueiredo, M. Guerra

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Abstract

BACKGROUND Semen cryopreservation is a biotechnology used frequently in animal production; however, there are some obstacles, such as those caused by high levels of reactive oxygen species (ROS). Moringa oleifera (MO) is known as a potent source of antioxidants and might be an important adjuvant. OBJECTIVE The objective of this study was to determine the effect of different concentrations of MO extract supplementation on goat semen cryopreservation efficiency. MATERIALS AND METHODS Ejaculates (n=6) from four goat breeders were pooled and diluted in skimmed milk (SM) or Tris-egg yolk (TEY)-based extenders and supplemented with different concentrations of MO extract (0, 1, 2 and 5 mg/mL). After the freeze-thaw cycle, sperm kinetics and viability were assessed. RESULTS With the SM extender, straightness, wobble and plasma membrane integrity were lower than in the control group (P < 0.05). With the TEY extender, wobble was lower in with 5 mg/mL MO extract than in the control group (P < 0.05). As regards sperm ultrastructure, evaluated by SEM, the MO extract, regardless of the diluent used, damaged the membrane of sperm cells in a dose-dependent manner. CONCLUSION The addition of aqueous extract of MO leaves in both diluents at all concentrations tested affects the parameters of sperm progressivity and damages the plasma membrane in a dose-dependent manner. DOI: 10.54680/fr23310110712.

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辣木叶水提物加入冷冻剂中会破坏山羊精子膜。

精子冷冻保存是一种经常用于动物生产的生物技术;然而，也有一些障碍，如活性氧(ROS)水平高引起的障碍。辣木(MO)被认为是抗氧化剂的有效来源，可能是一种重要的佐剂。目的研究添加不同浓度的MO提取物对山羊精液冷冻保存效率的影响。材料与方法将4个山羊种山羊的皂角酸盐(n=6)混合，用脱脂乳(SM)或三蛋黄(TEY)填充剂稀释，并添加不同浓度的MO提取物(0、1、2和5 mg/mL)。冻融循环后，评估精子动力学和活力。结果与对照组相比，使用SM扩展器后患者的直度、摆动度和质膜完整性均明显降低(P < 0.05)。在试验中，添加5 mg/mL MO提取物可显著降低小鼠的摇摆度(P < 0.05)。在精子超微结构方面，通过扫描电镜评估，无论使用何种稀释剂，MO提取物都以剂量依赖的方式破坏精子细胞膜。结论MO叶水提物在两种稀释剂中均对精子进行性参数有影响，且对质膜的损伤呈剂量依赖性。DOI: 10.54680 / fr23310110712。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.