Spectrophotometric Determination of Amprenavir by ComplexFormation in Bulk Drug and Formulation Samples

Abstract

A validate rapid, economical and sensitive visible spectrophotometric method has been developed for quantitative determination of amprenavir in bulk drug and formulation samples. This method is validated for irinotecan with chromogenic reagent 3-methyl benzothiazolinone hydrazine (MBTH). The calibration curve was linear over Beer’s concentration range of 25-350 µg/ml. The relative standard deviation is less than 1% and average recovery is above 99.80%. Efficient visible spectrophotometric detection at the respective absorption maxima enabled determination with no interference from tablet excipients .The proposed method is fast, sensitive, precise, accurate and efficient and can be used for analysis in quality control laboratories.