Isorhamnetin Alleviates the Depression Induced by Hindlimb Unloading in Rats

IF 1.3 4区工程技术 Q2 ENGINEERING, AEROSPACE

Microgravity Science and Technology Pub Date : 2023-08-31 DOI:10.1007/s12217-023-10070-z

Xiaoni Deng, Tingting Ren, Hao Zhang, Shuo Gao, Wenhui Yang, Jiaqi Zhang, Hong Yu, Xiang Jin, Hong Wang, Fan Li, Limin Zhai, Hafiz Muhammad Umer Farooq, Wenjuan Zhang, Airong Qian

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Abstract

Depression induced by weightlessness exposure in spaceflight has seriously affected astronauts’ psychology and flight missions, but the preventive and treatment methods remain limited. Here, we used systems pharmacology to identify the potential bioactive compounds from Hippophae rhamnoides L. (HR) for treating depression caused by weightlessness. First, isorhamnetin was screened out as a potential drug in HR for treating depression. Further, the therapeutic effect of isorhamnetin was investigated in depression induced by weightlessness using the hindlimb unloading (HLU) rat model. We found that treatment with isorhamnetin notably shortened immobility time during forced swimming tests and tail suspension tests in HLU rats. The hematoxylin-eosin staining results revealed that isorhamnetin could ameliorate morphological damage to the hippocampus of HLU-induced rats. Moreover, exposure to HLU caused increased corticosterone (CORT) and adrenocorticotropic hormone (ACTH) concentrations in serum. Administration with isorhamnetin for four weeks reduced the ACTH and CORT content in HLU rats. The 5-hydroxytryptamine and dopamine content in hippocampus were reduced in HLU rats, which were increased after isorhamnetin-treatment. Conclusively, isorhamnetin can alleviate the depression and hippocampus damage induced by weightlessness. Our study identified that isorhamnetin could be a natural bioactive drug for depression.

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异鼠李素减轻大鼠后肢卸荷所致抑郁

航天飞行中暴露在失重环境下引起的抑郁症严重影响了航天员的心理和飞行任务，但预防和治疗方法仍然有限。本研究利用系统药理学方法对沙棘(Hippophae rhamnoides L.， HR)中治疗失重所致抑郁症的潜在生物活性化合物进行了鉴定。首先，异鼠李素被筛选为HR治疗抑郁症的潜在药物。采用后肢卸荷(HLU)大鼠模型研究异鼠李素对失重所致抑郁的治疗作用。我们发现异鼠李素显著缩短了HLU大鼠在强迫游泳试验和悬尾试验中的静止时间。苏木精-伊红染色结果显示异鼠李素能改善hlu诱导大鼠海马的形态学损伤。此外，暴露于HLU导致血清中皮质酮(CORT)和促肾上腺皮质激素(ACTH)浓度升高。异鼠李素给药四周可降低HLU大鼠ACTH和CORT含量。HLU大鼠海马中5-羟色胺和多巴胺含量降低，异鼠李素处理后海马中5-羟色胺和多巴胺含量升高。因此，异鼠李素可以减轻失重引起的抑郁和海马损伤。我们的研究发现异鼠李素可能是一种治疗抑郁症的天然生物活性药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microgravity Science and Technology 工程技术-工程：宇航

CiteScore

3.50

自引率

44.40%

发文量

期刊介绍： Microgravity Science and Technology – An International Journal for Microgravity and Space Exploration Related Research is a is a peer-reviewed scientific journal concerned with all topics, experimental as well as theoretical, related to research carried out under conditions of altered gravity. Microgravity Science and Technology publishes papers dealing with studies performed on and prepared for platforms that provide real microgravity conditions (such as drop towers, parabolic flights, sounding rockets, reentry capsules and orbiting platforms), and on ground-based facilities aiming to simulate microgravity conditions on earth (such as levitrons, clinostats, random positioning machines, bed rest facilities, and micro-scale or neutral buoyancy facilities) or providing artificial gravity conditions (such as centrifuges). Data from preparatory tests, hardware and instrumentation developments, lessons learnt as well as theoretical gravity-related considerations are welcome. Included science disciplines with gravity-related topics are: − materials science − fluid mechanics − process engineering − physics − chemistry − heat and mass transfer − gravitational biology − radiation biology − exobiology and astrobiology − human physiology