Methodic Approach of Atomic-Force Microscopy (AFM) to Study Morphological Changes of Cells and Model Systems

IF 1.5 4区材料科学 Q4 MATERIALS SCIENCE, MULTIDISCIPLINARY

Micro & Nano Letters Pub Date : 2023-03-28 DOI:10.3390/micro3020026

V. Binyukov, E. Mil, L. Matienko, A. Albantova, A. Goloshchapov

引用次数: 1

Abstract

For the first time AFM (atomic-force microscopy) was used to record significant changes in the geometric parameters of the image of erythrocytes in vitro under conditions of glycolytic starvation (ATP (Adenosine triphosphate) deficiency). The difference in the action of antioxidants, phenosan K, and Ihfan-10 on erythrocytes that we detected with AFM seems to be mainly due to their difference in hydrophobicity. We used the AFM method to research the self-organization of the components of the active center of P450 (Porphyrin-450) metalloenzymes that are part of a class of hemoproteins with functions of affinity to molecular oxygen O2. Stable supramolecular nanostructures in the form of triangular prisms based on the iron porphyrin complex with amino acids due to self-assembly involving intermolecular hydrogen bonds were received. A possible scheme for the formation of such structures is proposed.

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原子力显微镜(AFM)研究细胞和模型系统形态变化的方法

首次使用原子力显微镜(AFM)记录了糖酵解饥饿(ATP (Adenosine triphosphate)缺乏)条件下红细胞体外图像几何参数的显著变化。我们用AFM检测到的抗氧化剂，phenosan K和Ihfan-10对红细胞作用的差异似乎主要是由于它们的疏水性不同。我们使用AFM方法研究了P450(卟啉-450)金属酶活性中心组分的自组织，P450金属酶是一类具有分子氧亲和功能的血红蛋白的一部分。通过分子间氢键的自组装，获得了基于铁卟啉与氨基酸配合物的稳定的三角棱柱状超分子纳米结构。提出了形成这种结构的一种可能方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Micro & Nano Letters 工程技术-材料科学：综合

CiteScore

3.30

自引率

0.00%

发文量

审稿时长

2.8 months

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