{"title":"Production of xylitol from D-xylulose by Mycobacterium smegmatis","authors":"Ken Izumori, Keiji Tuzaki","doi":"10.1016/0385-6380(88)90126-4","DOIUrl":null,"url":null,"abstract":"<div><p><em>Mycobacterium smegmatis</em> transformed <span>D</span>-xylulose to xylitol in washed cell reactions under aerobic and anaerobic conditions. The yield of xylitol reached about 70% in anaerobic conditions (in N<sub>2</sub>) by cells grown on media containing xylitol or <span>D</span>-mannitol. Cells immobilized with Ca-alginate had almost the same activity of xylitol production as washed cells.</p><p>Xylitol was produced from <span>D</span>-xylose using commercial immobilized <span>D</span>-xylose isomerase from <em>Bacillus coagulans</em> and immobilized cells of <em>M. smegmatis</em>. From 10 g of <span>D</span>-xylose, 4 g of xylitol was produced and 5 g of <span>D</span>-xylose remained in the reaction mixture; no <span>D</span>-xylulose was detected.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 1","pages":"Pages 33-36"},"PeriodicalIF":0.0000,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90126-4","citationCount":"47","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fermentation Technology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0385638088901264","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 47
Abstract
Mycobacterium smegmatis transformed D-xylulose to xylitol in washed cell reactions under aerobic and anaerobic conditions. The yield of xylitol reached about 70% in anaerobic conditions (in N2) by cells grown on media containing xylitol or D-mannitol. Cells immobilized with Ca-alginate had almost the same activity of xylitol production as washed cells.
Xylitol was produced from D-xylose using commercial immobilized D-xylose isomerase from Bacillus coagulans and immobilized cells of M. smegmatis. From 10 g of D-xylose, 4 g of xylitol was produced and 5 g of D-xylose remained in the reaction mixture; no D-xylulose was detected.
耻垢分枝杆菌在好氧和厌氧条件下将d -木糖糖转化为木糖醇。在含木糖醇或d -甘露醇培养基上生长的细胞，在厌氧条件下(N2)木糖醇的产率可达70%左右。用海藻酸钙固定的细胞与水洗细胞的木糖醇生产活性几乎相同。利用凝固芽孢杆菌固定化d -木糖异构酶和耻垢分枝杆菌固定化细胞，以d -木糖为原料制备木糖醇。10 g d -木糖生成4 g木糖醇，反应混合物中保留5 g d -木糖;未检测到d -木酮糖。
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